2a94
From Proteopedia
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| - | [[Image:2a94.gif|left|200px]] | + | [[Image:2a94.gif|left|200px]] |
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| - | '''Structure of Plasmodium falciparum lactate dehydrogenase complexed to APADH.''' | + | {{Structure |
| + | |PDB= 2a94 |SIZE=350|CAPTION= <scene name='initialview01'>2a94</scene>, resolution 1.500Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=AP0:ACETYL PYRIDINE ADENINE DINUCLEOTIDE, REDUCED'>AP0</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/L-lactate_dehydrogenase L-lactate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.27 1.1.1.27] | ||
| + | |GENE= LDH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of Plasmodium falciparum lactate dehydrogenase complexed to APADH.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2A94 is a [ | + | 2A94 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A94 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of lactate dehydrogenase from Plasmodium vivax: complexes with NADH and APADH., Chaikuad A, Fairweather V, Conners R, Joseph-Horne T, Turgut-Balik D, Brady RL, Biochemistry. 2005 Dec 13;44(49):16221-8. PMID:[http:// | + | Structure of lactate dehydrogenase from Plasmodium vivax: complexes with NADH and APADH., Chaikuad A, Fairweather V, Conners R, Joseph-Horne T, Turgut-Balik D, Brady RL, Biochemistry. 2005 Dec 13;44(49):16221-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16331982 16331982] |
[[Category: L-lactate dehydrogenase]] | [[Category: L-lactate dehydrogenase]] | ||
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:46:51 2008'' |
Revision as of 13:46, 20 March 2008
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| , resolution 1.500Å | |||||||
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| Ligands: | |||||||
| Gene: | LDH (Plasmodium falciparum) | ||||||
| Activity: | L-lactate dehydrogenase, with EC number 1.1.1.27 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Plasmodium falciparum lactate dehydrogenase complexed to APADH.
Overview
Malaria caused by Plasmodium vivax is a major cause of global morbidity and, in rare cases, mortality. Lactate dehydrogenase is an essential Plasmodium protein and, therefore, a potential antimalarial drug target. Ideally, drugs directed against this target would be effective against both major species of Plasmodium, P. falciparum and P. vivax. In this study, the crystal structure of the lactate dehydrogenase protein from P. vivax has been solved and is compared to the equivalent structure from the P. falciparum enzyme. The active sites and cofactor binding pockets of both enzymes are found to be highly similar and differentiate these enzymes from their human counterparts. These structures suggest effective inhibition of both enzymes should be readily achievable with a common inhibitor. The crystal structures of both enzymes have also been solved in complex with the synthetic cofactor APADH. The unusual cofactor binding site in these Plasmodium enzymes is found to readily accommodate both NADH and APADH, explaining why the Plasmodium enzymes retain enzymatic activity in the presence of this synthetic cofactor.
About this Structure
2A94 is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Structure of lactate dehydrogenase from Plasmodium vivax: complexes with NADH and APADH., Chaikuad A, Fairweather V, Conners R, Joseph-Horne T, Turgut-Balik D, Brady RL, Biochemistry. 2005 Dec 13;44(49):16221-8. PMID:16331982
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