2ab5
From Proteopedia
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| - | [[Image:2ab5.gif|left|200px]] | + | [[Image:2ab5.gif|left|200px]] |
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| - | '''bI3 LAGLIDADG Maturase''' | + | {{Structure |
| + | |PDB= 2ab5 |SIZE=350|CAPTION= <scene name='initialview01'>2ab5</scene>, resolution 2.200Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= cytochrome b intron bi3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''bI3 LAGLIDADG Maturase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2AB5 is a [ | + | 2AB5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB5 OCA]. |
==Reference== | ==Reference== | ||
| - | Evolution from DNA to RNA recognition by the bI3 LAGLIDADG maturase., Longo A, Leonard CW, Bassi GS, Berndt D, Krahn JM, Hall TM, Weeks KM, Nat Struct Mol Biol. 2005 Sep;12(9):779-87. Epub 2005 Aug 21. PMID:[http:// | + | Evolution from DNA to RNA recognition by the bI3 LAGLIDADG maturase., Longo A, Leonard CW, Bassi GS, Berndt D, Krahn JM, Hall TM, Weeks KM, Nat Struct Mol Biol. 2005 Sep;12(9):779-87. Epub 2005 Aug 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16116439 16116439] |
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: rna binding]] | [[Category: rna binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:47:41 2008'' |
Revision as of 13:47, 20 March 2008
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| , resolution 2.200Å | |||||||
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| Ligands: | |||||||
| Gene: | cytochrome b intron bi3 (Saccharomyces cerevisiae) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
bI3 LAGLIDADG Maturase
Overview
LAGLIDADG endonucleases bind across adjacent major grooves via a saddle-shaped surface and catalyze DNA cleavage. Some LAGLIDADG proteins, called maturases, facilitate splicing by group I introns, raising the issue of how a DNA-binding protein and an RNA have evolved to function together. In this report, crystallographic analysis shows that the global architecture of the bI3 maturase is unchanged from its DNA-binding homologs; in contrast, the endonuclease active site, dispensable for splicing facilitation, is efficiently compromised by a lysine residue replacing essential catalytic groups. Biochemical experiments show that the maturase binds a peripheral RNA domain 50 A from the splicing active site, exemplifying long-distance structural communication in a ribonucleoprotein complex. The bI3 maturase nucleic acid recognition saddle interacts at the RNA minor groove; thus, evolution from DNA to RNA function has been mediated by a switch from major to minor groove interaction.
About this Structure
2AB5 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Evolution from DNA to RNA recognition by the bI3 LAGLIDADG maturase., Longo A, Leonard CW, Bassi GS, Berndt D, Krahn JM, Hall TM, Weeks KM, Nat Struct Mol Biol. 2005 Sep;12(9):779-87. Epub 2005 Aug 21. PMID:16116439
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