2abw
From Proteopedia
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| - | [[Image:2abw.gif|left|200px]] | + | [[Image:2abw.gif|left|200px]] |
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| - | '''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)''' | + | {{Structure |
| + | |PDB= 2abw |SIZE=350|CAPTION= <scene name='initialview01'>2abw</scene>, resolution 1.620Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PG4:TETRAETHYLENE GLYCOL'>PG4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= Pdx2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum]) | ||
| + | }} | ||
| + | |||
| + | '''Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ABW is a [ | + | 2ABW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ABW OCA]. |
==Reference== | ==Reference== | ||
| - | Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:[http:// | + | Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16339145 16339145] |
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: vitamin b6]] | [[Category: vitamin b6]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:47:49 2008'' |
Revision as of 13:47, 20 March 2008
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| , resolution 1.620Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | Pdx2 (Plasmodium falciparum) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Glutaminase subunit of the plasmodial PLP synthase (Vitamin B6 biosynthesis)
Overview
Vitamin B6 is one of nature's most versatile cofactors. Most organisms synthesize vitamin B6 via a recently discovered pathway employing the proteins Pdx1 and Pdx2. Here we present an in-depth characterization of the respective orthologs from the malaria parasite, Plasmodium falciparum. Expression profiling of Pdx1 and -2 shows that blood-stage parasites indeed possess a functional vitamin B6 de novo biosynthesis. Recombinant Pdx1 and Pdx2 form a complex that functions as a glutamine amidotransferase with Pdx2 as the glutaminase and Pdx1 as pyridoxal-5 '-phosphate synthase domain. Complex formation is required for catalytic activity of either domain. Pdx1 forms a chimeric bi-enzyme with the bacterial YaaE, a Pdx2 ortholog, both in vivo and in vitro, although this chimera does not attain full catalytic activity, emphasizing that species-specific structural features govern the interaction between the protein partners of the PLP synthase complexes in different organisms. To gain insight into the activation mechanism of the parasite bi-enzyme complex, the three-dimensional structure of Pdx2 was determined at 1.62 A. The obstruction of the oxyanion hole indicates that Pdx2 is in a resting state and that activation occurs upon Pdx1-Pdx2 complex formation.
About this Structure
2ABW is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
Reference
Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights., Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Muller S, Macheroux P, Tews I, Kappes B, J Biol Chem. 2006 Feb 10;281(6):3633-41. Epub 2005 Dec 8. PMID:16339145
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