2acf
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| - | [[Image:2acf.gif|left|200px]] | + | [[Image:2acf.gif|left|200px]] |
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| - | '''Crystal structure of ADRP domain of SARS NSP3''' | + | {{Structure |
| + | |PDB= 2acf |SIZE=350|CAPTION= <scene name='initialview01'>2acf</scene>, resolution 1.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Crystal structure of ADRP domain of SARS NSP3''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2ACF is a [ | + | 2ACF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ACF OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1''-phosphate dephosphorylation by a conserved domain of nsP3., Saikatendu KS, Joseph JS, Subramanian V, Clayton T, Griffith M, Moy K, Velasquez J, Neuman BW, Buchmeier MJ, Stevens RC, Kuhn P, Structure. 2005 Nov;13(11):1665-75. PMID:[http:// | + | Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1''-phosphate dephosphorylation by a conserved domain of nsP3., Saikatendu KS, Joseph JS, Subramanian V, Clayton T, Griffith M, Moy K, Velasquez J, Neuman BW, Buchmeier MJ, Stevens RC, Kuhn P, Structure. 2005 Nov;13(11):1665-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16271890 16271890] |
[[Category: Sars coronavirus]] | [[Category: Sars coronavirus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: adrp domain]] | [[Category: adrp domain]] | ||
| - | [[Category: appr-1 | + | [[Category: appr-1 -p phosphatase]] |
[[Category: jcsg]] | [[Category: jcsg]] | ||
| - | [[Category: joint center for structural | + | [[Category: joint center for structural genomic]] |
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi-2]] | [[Category: psi-2]] | ||
[[Category: sars nsp-3]] | [[Category: sars nsp-3]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:47:59 2008'' |
Revision as of 13:47, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of ADRP domain of SARS NSP3
Overview
The crystal structure of a conserved domain of nonstructural protein 3 (nsP3) from severe acute respiratory syndrome coronavirus (SARS-CoV) has been solved by single-wavelength anomalous dispersion to 1.4 A resolution. The structure of this "X" domain, seen in many single-stranded RNA viruses, reveals a three-layered alpha/beta/alpha core with a macro-H2A-like fold. The putative active site is a solvent-exposed cleft that is conserved in its three structural homologs, yeast Ymx7, Archeoglobus fulgidus AF1521, and Er58 from E. coli. Its sequence is similar to yeast YBR022W (also known as Poa1P), a known phosphatase that acts on ADP-ribose-1-phosphate (Appr-1-p). The SARS nsP3 domain readily removes the 1 phosphate group from Appr-1-p in in vitro assays, confirming its phosphatase activity. Sequence and structure comparison of all known macro-H2A domains combined with available functional data suggests that proteins of this superfamily form an emerging group of nucleotide phosphatases that dephosphorylate Appr-1-p.
About this Structure
2ACF is a Single protein structure of sequence from Sars coronavirus. Full crystallographic information is available from OCA.
Reference
Structural basis of severe acute respiratory syndrome coronavirus ADP-ribose-1-phosphate dephosphorylation by a conserved domain of nsP3., Saikatendu KS, Joseph JS, Subramanian V, Clayton T, Griffith M, Moy K, Velasquez J, Neuman BW, Buchmeier MJ, Stevens RC, Kuhn P, Structure. 2005 Nov;13(11):1665-75. PMID:16271890
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