2alc
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2alc.gif|left|200px]] | + | [[Image:2alc.gif|left|200px]] |
| - | + | ||
| - | '''ETHANOL REGULON TRANSCRIPTIONAL ACTIVATOR DNA-BINDING DOMAIN FROM ASPERGILLUS NIDULANS''' | + | {{Structure |
| + | |PDB= 2alc |SIZE=350|CAPTION= <scene name='initialview01'>2alc</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= ALCR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=162425 Emericella nidulans]) | ||
| + | }} | ||
| + | |||
| + | '''ETHANOL REGULON TRANSCRIPTIONAL ACTIVATOR DNA-BINDING DOMAIN FROM ASPERGILLUS NIDULANS''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2ALC is a [ | + | 2ALC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ALC OCA]. |
==Reference== | ==Reference== | ||
| - | NMR solution structure of AlcR (1-60) provides insight in the unusual DNA binding properties of this zinc binuclear cluster protein., Cerdan R, Cahuzac B, Felenbok B, Guittet E, J Mol Biol. 2000 Jan 28;295(4):729-36. PMID:[http:// | + | NMR solution structure of AlcR (1-60) provides insight in the unusual DNA binding properties of this zinc binuclear cluster protein., Cerdan R, Cahuzac B, Felenbok B, Guittet E, J Mol Biol. 2000 Jan 28;295(4):729-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10656785 10656785] |
[[Category: Emericella nidulans]] | [[Category: Emericella nidulans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 22: | Line 31: | ||
[[Category: zinc binuclear cluster]] | [[Category: zinc binuclear cluster]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:51:03 2008'' |
Revision as of 13:51, 20 March 2008
| |||||||
| Ligands: | |||||||
| Gene: | ALCR (Emericella nidulans) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ETHANOL REGULON TRANSCRIPTIONAL ACTIVATOR DNA-BINDING DOMAIN FROM ASPERGILLUS NIDULANS
Overview
The three-dimensional structure of the DNA-binding domain (residues 1-60) of the ethanol regulon transcription factor AlcR from Aspergillus nidulans has been solved by NMR. This domain belongs to the zinc binuclear cluster class. Although the core of the protein is similar to previously characterized structures, consisting of two helices organized around a Zn(2)Cys(6 )motif, the present structure presents important variations, among them the presence of two supplementary helices. This structure gives new insight into the understanding of the AlcR specificities in DNA binding such as longer consensus half-sites, in vitro monomeric binding but in vivo multiple repeat transcriptional activation, either in direct or inverse orientations. The presence of additional contacts of the protein with its DNA target can be predicted from a model proposed for the interaction with the consensus DNA target. The clustering of accessible negative charges on helix 2 delineates a possible interaction site for other determinants of the transcriptional machinery, responsible for the fine tuning of the selection of the AlcR cognate sites.
About this Structure
2ALC is a Single protein structure of sequence from Emericella nidulans. Full crystallographic information is available from OCA.
Reference
NMR solution structure of AlcR (1-60) provides insight in the unusual DNA binding properties of this zinc binuclear cluster protein., Cerdan R, Cahuzac B, Felenbok B, Guittet E, J Mol Biol. 2000 Jan 28;295(4):729-36. PMID:10656785
Page seeded by OCA on Thu Mar 20 15:51:03 2008
