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2xzf

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<StructureSection load='2xzf' size='340' side='right' caption='[[2xzf]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='2xzf' size='340' side='right' caption='[[2xzf]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2xzf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris Lactococcus lactis subsp. cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZF OCA]. <br>
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<table><tr><td colspan='2'>[[2xzf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._cremoris Lactococcus lactis subsp. cremoris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XZF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XZF FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=VET:[(1R,2S,4R)-2-HYDROXY-4-[(5R)-5-HYDROXY-5-METHYL-2,4-DIOXO-IMIDAZOLIDIN-1-YL]CYCLOPENTYL]METHYL+DIHYDROGEN+PHOSPHATE'>VET</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=VET:[(1R,2S,4R)-2-HYDROXY-4-[(5R)-5-HYDROXY-5-METHYL-2,4-DIOXO-IMIDAZOLIDIN-1-YL]CYCLOPENTYL]METHYL+DIHYDROGEN+PHOSPHATE'>VET</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pji|1pji]], [[1pm5|1pm5]], [[1xc8|1xc8]], [[1tdz|1tdz]], [[1pjj|1pjj]], [[1kfv|1kfv]], [[1nnj|1nnj]]</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pji|1pji]], [[1pm5|1pm5]], [[1xc8|1xc8]], [[1tdz|1tdz]], [[1pjj|1pjj]], [[1kfv|1kfv]], [[1nnj|1nnj]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-formamidopyrimidine_glycosylase DNA-formamidopyrimidine glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.23 3.2.2.23] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xzf RCSB], [http://www.ebi.ac.uk/pdbsum/2xzf PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xzf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xzf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xzf RCSB], [http://www.ebi.ac.uk/pdbsum/2xzf PDBsum]</span></td></tr>
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<table>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/FPG_LACLC FPG_LACLC]] Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.<ref>PMID:7704272</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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5-Hydroxy-5-methylhydantoin DNA lesion, a molecular trap for DNA glycosylases.,Le Bihan YV, Angeles Izquierdo M, Coste F, Aller P, Culard F, Gehrke TH, Essalhi K, Carell T, Castaing B Nucleic Acids Res. 2011 Aug;39(14):6277-90. Epub 2011 Apr 12. PMID:21486746<ref>PMID:21486746</ref>
5-Hydroxy-5-methylhydantoin DNA lesion, a molecular trap for DNA glycosylases.,Le Bihan YV, Angeles Izquierdo M, Coste F, Aller P, Culard F, Gehrke TH, Essalhi K, Carell T, Castaing B Nucleic Acids Res. 2011 Aug;39(14):6277-90. Epub 2011 Apr 12. PMID:21486746<ref>PMID:21486746</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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==See Also==
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*[[DNA glycosylase|DNA glycosylase]]
== References ==
== References ==
<references/>
<references/>
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[[Category: DNA-formamidopyrimidine glycosylase]]
[[Category: DNA-formamidopyrimidine glycosylase]]
[[Category: Lactococcus lactis subsp. cremoris]]
[[Category: Lactococcus lactis subsp. cremoris]]
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[[Category: Aller, P.]]
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[[Category: Aller, P]]
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[[Category: Carrel, T.]]
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[[Category: Carrel, T]]
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[[Category: Castaing, B.]]
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[[Category: Castaing, B]]
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[[Category: Coste, F.]]
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[[Category: Coste, F]]
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[[Category: Culard, F.]]
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[[Category: Culard, F]]
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[[Category: Essalhi, K.]]
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[[Category: Essalhi, K]]
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[[Category: Gehrke, T H.]]
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[[Category: Gehrke, T H]]
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[[Category: Izquierdo, M A.]]
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[[Category: Izquierdo, M A]]
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[[Category: Lebihan, Y V.]]
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[[Category: Lebihan, Y V]]
[[Category: Hydrolase-dna complex]]
[[Category: Hydrolase-dna complex]]

Revision as of 00:49, 25 December 2014

CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THE WILD-TYPE LACTOCOCCUS LACTIS FPG (MUTM) AND AN OXIDIZED PYRIMIDINE CONTAINING DNA AT 293K

2xzf, resolution 1.80Å

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