2ap5
From Proteopedia
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| - | [[Image:2ap5.gif|left|200px]] | + | [[Image:2ap5.gif|left|200px]] |
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| - | '''Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, Average Structure''' | + | {{Structure |
| + | |PDB= 2ap5 |SIZE=350|CAPTION= <scene name='initialview01'>2ap5</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, Average Structure''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2AP5 is a [ | + | 2AP5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AP5 OCA]. |
==Reference== | ==Reference== | ||
| - | The global structures of a wild-type and poorly functional plant luteoviral mRNA pseudoknot are essentially identical., Cornish PV, Stammler SN, Giedroc DP, RNA. 2006 Nov;12(11):1959-69. Epub 2006 Sep 25. PMID:[http:// | + | The global structures of a wild-type and poorly functional plant luteoviral mRNA pseudoknot are essentially identical., Cornish PV, Stammler SN, Giedroc DP, RNA. 2006 Nov;12(11):1959-69. Epub 2006 Sep 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17000902 17000902] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Cornish, P V.]] | [[Category: Cornish, P V.]] | ||
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[[Category: rna pseudoknot; frameshifting]] | [[Category: rna pseudoknot; frameshifting]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:52:12 2008'' |
Revision as of 13:52, 20 March 2008
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Solution Structure of the C27A ScYLV P1-P2 Frameshifting Pseudoknot, Average Structure
Overview
The helical junction region of a -1 frameshift stimulating hairpin-type mRNA pseudoknot from sugarcane yellow leaf virus (ScYLV) is characterized by a novel C27.(G7-C14) loop 2-stem 1 minor groove base triple, which is stacked on a C8+.(G12-C28) loop 1-stem 2 major groove base triple. Substitution of C27 with adenosine reduces frameshifting efficiency to a level just twofold above the slip-site alone. Here, we show that the global structure of the C27A ScYLV RNA is nearly indistinguishable from the wild-type counterpart, despite the fact that the helical junction region is altered and incorporates the anticipated isostructural A27.(G7-C14) minor groove base triple. This interaction mediates a 2.3-A displacement of C8+ driven by an A27 N6-C8+ O2 hydrogen bond as part of an A(n-1).C+.G-Cn base quadruple. The helical junction regions of the C27A ScYLV and the beet western yellows virus (BWYV) pseudoknots are essentially superimposable, the latter of which contains an analogous A25.(G7-C14) minor groove base triple. These results reveal that the global ground-state structure is not strongly correlated with frameshift stimulation and point to a reduced thermodynamic stability and/or enhanced kinetic lability that derives from an altered helical junction architecture in the C27A ScYLV RNA as a significant determinant for setting frameshifting efficiencies in plant luteoviral mRNA pseudoknots.
About this Structure
2AP5 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The global structures of a wild-type and poorly functional plant luteoviral mRNA pseudoknot are essentially identical., Cornish PV, Stammler SN, Giedroc DP, RNA. 2006 Nov;12(11):1959-69. Epub 2006 Sep 25. PMID:17000902
Page seeded by OCA on Thu Mar 20 15:52:12 2008
