2apo
From Proteopedia
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| - | [[Image:2apo.gif|left|200px]] | + | [[Image:2apo.gif|left|200px]] |
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| - | '''Crystal Structure of the Methanococcus jannaschii Cbf5 Nop10 Complex''' | + | {{Structure |
| + | |PDB= 2apo |SIZE=350|CAPTION= <scene name='initialview01'>2apo</scene>, resolution 1.95Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= truB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii]), NOP10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Methanococcus jannaschii Cbf5 Nop10 Complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2APO is a [ | + | 2APO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2APO OCA]. |
==Reference== | ==Reference== | ||
| - | The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs., Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR, Nat Struct Mol Biol. 2005 Dec;12(12):1101-7. Epub 2005 Nov 15. PMID:[http:// | + | The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs., Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR, Nat Struct Mol Biol. 2005 Dec;12(12):1101-7. Epub 2005 Nov 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16286935 16286935] |
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: zinc ribbon]] | [[Category: zinc ribbon]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:52:21 2008'' |
Revision as of 13:52, 20 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | truB (Methanocaldococcus jannaschii), NOP10 (Methanocaldococcus jannaschii) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Methanococcus jannaschii Cbf5 Nop10 Complex
Overview
Box H/ACA ribonucleoprotein particles (RNPs) catalyze RNA pseudouridylation and direct processing of ribosomal RNA, and are essential architectural components of vertebrate telomerases. H/ACA RNPs comprise four proteins and a multihelical RNA. Two proteins, Cbf5 and Nop10, suffice for basal enzymatic activity in an archaeal in vitro system. We now report their cocrystal structure at 1.95-A resolution. We find that archaeal Cbf5 can assemble with yeast Nop10 and with human telomerase RNA, consistent with the high sequence identity of the RNP components between archaea and eukarya. Thus, the Cbf5-Nop10 architecture is phylogenetically conserved. The structure shows how Nop10 buttresses the active site of Cbf5, and it reveals two basic troughs that bidirectionally extend the active site cleft. Mutagenesis results implicate an adjacent basic patch in RNA binding. This tripartite RNA-binding surface may function as a molecular bracket that organizes the multihelical H/ACA and telomerase RNAs.
About this Structure
2APO is a Protein complex structure of sequences from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
The Cbf5-Nop10 complex is a molecular bracket that organizes box H/ACA RNPs., Hamma T, Reichow SL, Varani G, Ferre-D'Amare AR, Nat Struct Mol Biol. 2005 Dec;12(12):1101-7. Epub 2005 Nov 15. PMID:16286935
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