4g37

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[[Image:4g37.png|left|200px]]
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==Structure of cross-linked firefly luciferase in second catalytic conformation==
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<StructureSection load='4g37' size='340' side='right' caption='[[4g37]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4g37]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G37 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4G37 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SLU:5-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL]+ADENOSINE'>SLU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XLX:4,4-(ETHYLENEDIIMINO)BIS[4-OXOBUTYRATE]'>XLX</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lci|1lci]], [[3iep|3iep]], [[2d1s|2d1s]], [[4g36|4g36]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Photinus-luciferin_4-monooxygenase_(ATP-hydrolyzing) Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.12.7 1.13.12.7] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4g37 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g37 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4g37 RCSB], [http://www.ebi.ac.uk/pdbsum/4g37 PDBsum]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Beetle luciferases catalyze a two-step reaction that includes the initial adenylation of the luciferin substrate, followed by an oxidative decarboxylation that ultimately produces light. Evidence for homologous acyl-CoA synthetases supports a domain alternation catalytic mechanism in which these enzymes' C-terminal domain rotates by approximately 140 degrees to adopt two conformations that are used to catalyze the two partial reactions. While many structures exist of acyl-CoA synthetases in both conformations, to date only biochemical evidence supports domain alternation with luciferase. We have determined the structure of a cross-linked luciferase enzyme that is trapped in the second conformation. This new structure supports the role of the second catalytic conformation and provides insights into the biochemical mechanism of the luciferase oxidative step.
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{{STRUCTURE_4g37| PDB=4g37 | SCENE= }}
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Crystal Structure of Firefly Luciferase in a Second Catalytic Conformation Supports a Domain Alternation Mechanism.,Sundlov JA, Fontaine DM, Southworth TL, Branchini BR, Gulick AM Biochemistry. 2012 Aug 6. PMID:22852753<ref>PMID:22852753</ref>
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===Structure of cross-linked firefly luciferase in second catalytic conformation===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_22852753}}
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==See Also==
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*[[Luciferase|Luciferase]]
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==About this Structure==
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== References ==
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[[4g37]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Photinus_pyralis Photinus pyralis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G37 OCA].
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<references/>
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__TOC__
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==Reference==
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</StructureSection>
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<ref group="xtra">PMID:022852753</ref><references group="xtra"/>
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[[Category: Photinus pyralis]]
[[Category: Photinus pyralis]]
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[[Category: Branchini, B R.]]
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[[Category: Branchini, B R]]
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[[Category: Gulick, A M.]]
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[[Category: Gulick, A M]]
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[[Category: Sundlov, J A.]]
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[[Category: Sundlov, J A]]
[[Category: Adenylating enzyme]]
[[Category: Adenylating enzyme]]
[[Category: Anl superfamily]]
[[Category: Anl superfamily]]

Revision as of 01:20, 25 December 2014

Structure of cross-linked firefly luciferase in second catalytic conformation

4g37, resolution 2.40Å

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