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2mkc

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Revision as of 01:27, 25 December 2014

Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex

Structural highlights

2mkc is a 3 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[IST3_YEAST] Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.^[1] ^[2] ^[3] [CWC26_YEAST] Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal.^[4] ^[5] ^[6] ^[7] [PML1_YEAST] Required for efficient splicing and pre-mRNA nuclear retention.^[8]

Publication Abstract from PubMed

The precursor mRNA (pre-mRNA) retention and splicing (RES) complex is a spliceosomal complex that is present in yeast and humans and is important for RNA splicing and retention of unspliced pre-mRNA. Here, we present the solution NMR structure of the RES core complex from Saccharomyces cerevisiae. Complex formation leads to an intricate folding of three components-Snu17p, Bud13p and Pml1p-that stabilizes the RNA-recognition motif (RRM) fold of Snu17p and increases binding affinity in tertiary interactions between the components by more than 100-fold compared to that in binary interactions. RES interacts with pre-mRNA within the spliceosome, and through the assembly of the RES core complex RNA binding efficiency is increased. The three-dimensional structure of the RES core complex highlights the importance of cooperative folding and binding in the functional organization of the spliceosome.

Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.,Wysoczanski P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Luhrmann R, Becker S, Zweckstetter M Nat Struct Mol Biol. 2014 Sep 14. doi: 10.1038/nsmb.2889. PMID:25218446^[9]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gottschalk A, Bartels C, Neubauer G, Luhrmann R, Fabrizio P. A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly. Mol Cell Biol. 2001 May;21(9):3037-46. PMID:11287609 doi:http://dx.doi.org/10.1128/MCB.21.9.3037-3046.2001
↑ Dziembowski A, Ventura AP, Rutz B, Caspary F, Faux C, Halgand F, Laprevote O, Seraphin B. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004 Dec 8;23(24):4847-56. Epub 2004 Nov 25. PMID:15565172 doi:http://dx.doi.org/7600482
↑ Spingola M, Armisen J, Ares M Jr. Mer1p is a modular splicing factor whose function depends on the conserved U2 snRNP protein Snu17p. Nucleic Acids Res. 2004 Feb 18;32(3):1242-50. Print 2004. PMID:14973223 doi:http://dx.doi.org/10.1093/nar/gkh281
↑ Zahner JE, Harkins HA, Pringle JR. Genetic analysis of the bipolar pattern of bud site selection in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 1996 Apr;16(4):1857-70. PMID:8657162
↑ Ni L, Snyder M. A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell. 2001 Jul;12(7):2147-70. PMID:11452010
↑ Vincent K, Wang Q, Jay S, Hobbs K, Rymond BC. Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing. Genetics. 2003 Jul;164(3):895-907. PMID:12871902
↑ Dziembowski A, Ventura AP, Rutz B, Caspary F, Faux C, Halgand F, Laprevote O, Seraphin B. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004 Dec 8;23(24):4847-56. Epub 2004 Nov 25. PMID:15565172 doi:http://dx.doi.org/7600482
↑ Dziembowski A, Ventura AP, Rutz B, Caspary F, Faux C, Halgand F, Laprevote O, Seraphin B. Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing. EMBO J. 2004 Dec 8;23(24):4847-56. Epub 2004 Nov 25. PMID:15565172 doi:http://dx.doi.org/7600482
↑ Wysoczanski P, Schneider C, Xiang S, Munari F, Trowitzsch S, Wahl MC, Luhrmann R, Becker S, Zweckstetter M. Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Nat Struct Mol Biol. 2014 Sep 14. doi: 10.1038/nsmb.2889. PMID:25218446 doi:http://dx.doi.org/10.1038/nsmb.2889

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mkc"

@@ Line 5: / Line 5: @@
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mkc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mkc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2mkc RCSB], [http://www.ebi.ac.uk/pdbsum/2mkc PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/IST3_YEAST IST3_YEAST]] Required for pre-mRNA splicing and spliceosome assembly. As part of the pre-mRNA retention and splicing (RES) complex, required for nuclear pre-mRNA retention and efficient splicing. Required for MER1-activated splicing.<ref>PMID:11287609</ref> <ref>PMID:15565172</ref> <ref>PMID:14973223</ref>  [[http://www.uniprot.org/uniprot/CWC26_YEAST CWC26_YEAST]] Required for efficient splicing and pre-mRNA nuclear retention. May also be involved in positioning the proximal bud pole signal.<ref>PMID:8657162</ref> <ref>PMID:11452010</ref> <ref>PMID:12871902</ref> <ref>PMID:15565172</ref>  [[http://www.uniprot.org/uniprot/PML1_YEAST PML1_YEAST]] Required for efficient splicing and pre-mRNA nuclear retention.<ref>PMID:15565172</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 __TOC__
 </StructureSection>
-[[Category: Becker, S.]]
+[[Category: Becker, S]]
-[[Category: Luhrmann, R.]]
+[[Category: Luhrmann, R]]
-[[Category: Munari, F.]]
+[[Category: Munari, F]]
-[[Category: Schneider, C.]]
+[[Category: Schneider, C]]
-[[Category: Trowitzsch, S.]]
+[[Category: Trowitzsch, S]]
-[[Category: Wahl, M C.]]
+[[Category: Wahl, M C]]
-[[Category: Wysoczanski, P.]]
+[[Category: Wysoczanski, P]]
-[[Category: Xiang, S.]]
+[[Category: Xiang, S]]
-[[Category: Zweckstetter, M.]]
+[[Category: Zweckstetter, M]]
 [[Category: Bud13p]]
 [[Category: Cooperativity]]

2mkc

From Proteopedia

Revision as of 01:27, 25 December 2014

Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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