2ass

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[[Image:2ass.gif|left|200px]]<br /><applet load="2ass" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2ass.gif|left|200px]]
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caption="2ass, resolution 3.00&Aring;" />
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'''Crystal structure of the Skp1-Skp2-Cks1 complex'''<br />
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{{Structure
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|PDB= 2ass |SIZE=350|CAPTION= <scene name='initialview01'>2ass</scene>, resolution 3.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>
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|ACTIVITY=
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|GENE= SKP1A, EMC19, OCP2, SKP1, TCEB1L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SKP2, FBXL1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CKS1, CKS1B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''Crystal structure of the Skp1-Skp2-Cks1 complex'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2ASS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=BEN:'>BEN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ASS OCA].
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2ASS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ASS OCA].
==Reference==
==Reference==
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Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase., Hao B, Zheng N, Schulman BA, Wu G, Miller JJ, Pagano M, Pavletich NP, Mol Cell. 2005 Oct 7;20(1):9-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16209941 16209941]
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Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase., Hao B, Zheng N, Schulman BA, Wu G, Miller JJ, Pagano M, Pavletich NP, Mol Cell. 2005 Oct 7;20(1):9-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16209941 16209941]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: scf]]
[[Category: scf]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:30:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:53:31 2008''

Revision as of 13:53, 20 March 2008

Image:2ass.gif


PDB ID 2ass

Drag the structure with the mouse to rotate
, resolution 3.00Å
Ligands: and
Gene: SKP1A, EMC19, OCP2, SKP1, TCEB1L (Homo sapiens), SKP2, FBXL1 (Homo sapiens), CKS1, CKS1B (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the Skp1-Skp2-Cks1 complex


Overview

The ubiquitin-mediated proteolysis of the Cdk2 inhibitor p27(Kip1) plays a central role in cell cycle progression, and enhanced degradation of p27(Kip1) is associated with many common cancers. Proteolysis of p27(Kip1) is triggered by Thr187 phosphorylation, which leads to the binding of the SCF(Skp2) (Skp1-Cul1-Rbx1-Skp2) ubiquitin ligase complex. Unlike other known SCF substrates, p27(Kip1) ubiquitination also requires the accessory protein Cks1. The crystal structure of the Skp1-Skp2-Cks1 complex bound to a p27(Kip1) phosphopeptide shows that Cks1 binds to the leucine-rich repeat (LRR) domain and C-terminal tail of Skp2, whereas p27(Kip1) binds to both Cks1 and Skp2. The phosphorylated Thr187 side chain of p27(Kip1) is recognized by a Cks1 phosphate binding site, whereas the side chain of an invariant Glu185 inserts into the interface between Skp2 and Cks1, interacting with both. The structure and biochemical data support the proposed model that Cdk2-cyclin A contributes to the recruitment of p27(Kip1) to the SCF(Skp2)-Cks1 complex.

About this Structure

2ASS is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis of the Cks1-dependent recognition of p27(Kip1) by the SCF(Skp2) ubiquitin ligase., Hao B, Zheng N, Schulman BA, Wu G, Miller JJ, Pagano M, Pavletich NP, Mol Cell. 2005 Oct 7;20(1):9-19. PMID:16209941

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