2auj
From Proteopedia
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| - | [[Image:2auj.gif|left|200px]] | + | [[Image:2auj.gif|left|200px]] |
| - | + | ||
| - | '''Structure of Thermus aquaticus RNA polymerase beta'-subunit insert''' | + | {{Structure |
| + | |PDB= 2auj |SIZE=350|CAPTION= <scene name='initialview01'>2auj</scene>, resolution 2.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | ||
| + | |GENE= rpoC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus]) | ||
| + | }} | ||
| + | |||
| + | '''Structure of Thermus aquaticus RNA polymerase beta'-subunit insert''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2AUJ is a [ | + | 2AUJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AUJ OCA]. |
==Reference== | ==Reference== | ||
| - | Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase beta' subunit., Chlenov M, Masuda S, Murakami KS, Nikiforov V, Darst SA, Mustaev A, J Mol Biol. 2005 Oct 14;353(1):138-54. PMID:[http:// | + | Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase beta' subunit., Chlenov M, Masuda S, Murakami KS, Nikiforov V, Darst SA, Mustaev A, J Mol Biol. 2005 Oct 14;353(1):138-54. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16154587 16154587] |
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sandwich-barrel hybrid motif]] | [[Category: sandwich-barrel hybrid motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:53:59 2008'' |
Revision as of 13:54, 20 March 2008
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| , resolution 2.7Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | rpoC (Thermus aquaticus) | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Thermus aquaticus RNA polymerase beta'-subunit insert
Overview
The large beta and beta' subunits of the bacterial core RNA polymerase (RNAP) are highly conserved throughout evolution. Nevertheless, large sequence insertions in beta and beta' characterize specific evolutionary lineages of bacteria. The Thermus aquaticus RNAP beta' subunit contains a 283 residue insert between conserved regions A and B that is found in only four bacterial species. The Escherichia coli RNAP beta' subunit contains a 188 residue insert in the middle of conserved region G that is found in a wide range of bacterial species. Here, we present structural studies of these two beta' insertions. We show that the inserts comprise repeats of a previously characterized fold, the sandwich-barrel hybrid motif (as predicted from previous sequence analysis) and that the inserts serve significant roles in facilitating protein/protein and/or protein/nucleic acid interactions.
About this Structure
2AUJ is a Single protein structure of sequence from Thermus aquaticus. Full crystallographic information is available from OCA.
Reference
Structure and function of lineage-specific sequence insertions in the bacterial RNA polymerase beta' subunit., Chlenov M, Masuda S, Murakami KS, Nikiforov V, Darst SA, Mustaev A, J Mol Biol. 2005 Oct 14;353(1):138-54. PMID:16154587
Page seeded by OCA on Thu Mar 20 15:53:59 2008
