2xyv
From Proteopedia
(Difference between revisions)
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<StructureSection load='2xyv' size='340' side='right' caption='[[2xyv]], [[Resolution|resolution]] 2.06Å' scene=''> | <StructureSection load='2xyv' size='340' side='right' caption='[[2xyv]], [[Resolution|resolution]] 2.06Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2xyv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XYV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2xyv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvhsa Cvhsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XYV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XYV FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2d2d|2d2d]], [[2ahm|2ahm]], [[1p9t|1p9t]], [[1uk3|1uk3]], [[2xyr|2xyr]], [[1wof|1wof]], [[2amd|2amd]], [[2a5i|2a5i]], [[2v6n|2v6n]], [[2bx4|2bx4]], [[2acf|2acf]], [[2xyq|2xyq]], [[1uk4|1uk4]], [[1z1i|1z1i]], [[1o5s|1o5s]], [[2a5k|2a5k]], [[2c3s|2c3s]], [[1sxf|1sxf]], [[1uj1|1uj1]], [[1uk2|1uk2]], [[2amq|2amq]], [[2bx3|2bx3]], [[1pa5|1pa5]], [[1puk|1puk]], [[1uw7|1uw7]], [[1p76|1p76]], [[1q1x|1q1x]], [[2a5a|2a5a]], [[1ysy|1ysy]], [[1z1j|1z1j]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2d2d|2d2d]], [[2ahm|2ahm]], [[1p9t|1p9t]], [[1uk3|1uk3]], [[2xyr|2xyr]], [[1wof|1wof]], [[2amd|2amd]], [[2a5i|2a5i]], [[2v6n|2v6n]], [[2bx4|2bx4]], [[2acf|2acf]], [[2xyq|2xyq]], [[1uk4|1uk4]], [[1z1i|1z1i]], [[1o5s|1o5s]], [[2a5k|2a5k]], [[2c3s|2c3s]], [[1sxf|1sxf]], [[1uj1|1uj1]], [[1uk2|1uk2]], [[2amq|2amq]], [[2bx3|2bx3]], [[1pa5|1pa5]], [[1puk|1puk]], [[1uw7|1uw7]], [[1p76|1p76]], [[1q1x|1q1x]], [[2a5a|2a5a]], [[1ysy|1ysy]], [[1z1j|1z1j]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xyv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xyv RCSB], [http://www.ebi.ac.uk/pdbsum/2xyv PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xyv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xyv RCSB], [http://www.ebi.ac.uk/pdbsum/2xyv PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/R1AB_CVHSA R1AB_CVHSA]] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products (By similarity).<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity). Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G). Activity of helicase is dependent on magnesium.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> Nsp9 is a ssRNA-binding protein.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond.<ref>PMID:17024178</ref> <ref>PMID:17692280</ref> <ref>PMID:19369340</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Cvhsa]] | ||
[[Category: Bouvet, M]] | [[Category: Bouvet, M]] | ||
[[Category: Canard, B]] | [[Category: Canard, B]] | ||
Revision as of 01:45, 25 December 2014
Crystal structure of the nsp16 nsp10 SARS coronavirus complex
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