2axe

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2axe.gif|left|200px]]<br /><applet load="2axe" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:2axe.gif|left|200px]]
-
caption="2axe, resolution 1.80&Aring;" />
+
 
-
'''IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS'''<br />
+
{{Structure
 +
|PDB= 2axe |SIZE=350|CAPTION= <scene name='initialview01'>2axe</scene>, resolution 1.80&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
 +
|ACTIVITY= [http://en.wikipedia.org/wiki/Acetylesterase Acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.6 3.1.1.6]
 +
|GENE=
 +
}}
 +
 
 +
'''IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
2AXE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_purpurogenum Penicillium purpurogenum] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetylesterase Acetylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.6 3.1.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXE OCA].
+
2AXE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Penicillium_purpurogenum Penicillium purpurogenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXE OCA].
==Reference==
==Reference==
-
Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase., Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089308 10089308]
+
Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase., Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089308 10089308]
[[Category: Acetylesterase]]
[[Category: Acetylesterase]]
[[Category: Penicillium purpurogenum]]
[[Category: Penicillium purpurogenum]]
Line 27: Line 36:
[[Category: esterase]]
[[Category: esterase]]
[[Category: hydrolase]]
[[Category: hydrolase]]
-
[[Category: iodotyrosines]]
+
[[Category: iodotyrosine]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:32:01 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:54:59 2008''

Revision as of 13:55, 20 March 2008

Image:2axe.gif


PDB ID 2axe

Drag the structure with the mouse to rotate
, resolution 1.80Å
Ligands:
Activity: Acetylesterase, with EC number 3.1.1.6
Coordinates: save as pdb, mmCIF, xml



IODINATED COMPLEX OF ACETYL XYLAN ESTERASE AT 1.80 ANGSTROMS


Overview

Enzymatic and non-enzymatic iodination of the amino acid tyrosine is a well known phenomenon. The iodination technique has been widely used for labeling proteins. Using high-resolution X-ray crystallographic techniques, the chemical and three-dimensional structures of iodotyrosines formed by non-enzymatic incorporation of I atoms into tyrosine residues of a crystalline protein are described. Acetylxylan esterase (AXE II; 207 amino-acid residues) from Penicillium purpurogenum has substrate specificities towards acetate esters of D-xylopyranose residues in xylan and belongs to a new class of alpha/beta hydrolases. The crystals of the enzyme are highly ordered, tightly packed and diffract to better than sub-angstrom resolution at 85 K. The iodination technique has been utilized to prepare an isomorphous derivative of the AXE II crystal. The structure of the enzyme determined at 1.10 A resolution exclusively by normal and anomalous scattering from I atoms, along with the structure of the iodinated complex at 1.80 A resolution, demonstrate the formation of covalent bonds between I atoms and C atoms at ortho positions to the hydroxyl groups of two tyrosyl moieties, yielding iodotyrosines.

About this Structure

2AXE is a Single protein structure of sequence from Penicillium purpurogenum. Full crystallographic information is available from OCA.

Reference

Determination of a protein structure by iodination: the structure of iodinated acetylxylan esterase., Ghosh D, Erman M, Sawicki M, Lala P, Weeks DR, Li N, Pangborn W, Thiel DJ, Jornvall H, Gutierrez R, Eyzaguirre J, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):779-84. PMID:10089308

Page seeded by OCA on Thu Mar 20 15:54:59 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2axe"
Personal tools