2b2c
From Proteopedia
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| - | [[Image:2b2c.gif|left|200px]] | + | [[Image:2b2c.gif|left|200px]] |
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| - | '''Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase''' | + | {{Structure |
| + | |PDB= 2b2c |SIZE=350|CAPTION= <scene name='initialview01'>2b2c</scene>, resolution 2.500Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2B2C is a [ | + | 2B2C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2C OCA]. |
==Reference== | ==Reference== | ||
| - | Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase., Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S, FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:[http:// | + | Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase., Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S, FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16226262 16226262] |
[[Category: Caenorhabditis elegans]] | [[Category: Caenorhabditis elegans]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: beta-alpha]] | [[Category: beta-alpha]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:56:42 2008'' |
Revision as of 13:56, 20 March 2008
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| , resolution 2.500Å | |||||||
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| Activity: | Spermidine synthase, with EC number 2.5.1.16 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Cloning, expression, characterisation and three- dimensional structure determination of the Caenorhabditis elegans spermidine synthase
Overview
The polyamine synthesis enzyme spermidine synthase (SPDS) has been cloned from the model nematode Caenorhabditis elegans. Biochemical characterisation of the recombinantly expressed protein revealed a high degree of similarity to other eukaryotic SPDS with the exception of a low affinity towards the substrate decarboxylated S-adenosylmethionine (Km = 110 microM) and a less pronounced feedback inhibition by the second reaction product 5'-methylthioadenosine (IC50 = 430 microM). The C. elegans protein that carries a nematode-specific insertion of 27 amino acids close to its N-terminus was crystallized, leading to the first X-ray structure of a dimeric eukaryotic SPDS.
About this Structure
2B2C is a Single protein structure of sequence from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Cloning, expression, characterisation and three-dimensional structure determination of Caenorhabditis elegans spermidine synthase., Dufe VT, Luersen K, Eschbach ML, Haider N, Karlberg T, Walter RD, Al-Karadaghi S, FEBS Lett. 2005 Nov 7;579(27):6037-43. Epub 2005 Oct 5. PMID:16226262
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