1o4z
From Proteopedia
(Difference between revisions)
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1o4z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Zobellia_galactanivorans Zobellia galactanivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O4Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O4Z FirstGlance]. <br> | <table><tr><td colspan='2'>[[1o4z]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Zobellia_galactanivorans Zobellia galactanivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O4Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1O4Z FirstGlance]. <br> | ||
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>< | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">agaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63186 Zobellia galactanivorans])</td></tr> | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">agaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63186 Zobellia galactanivorans])</td></tr> |
| - | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr> | + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-agarase Beta-agarase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.81 3.2.1.81] </span></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o4z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o4z RCSB], [http://www.ebi.ac.uk/pdbsum/1o4z PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1o4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o4z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1o4z RCSB], [http://www.ebi.ac.uk/pdbsum/1o4z PDBsum]</span></td></tr> |
| - | <table> | + | </table> |
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/Q9RGX8_9FLAO Q9RGX8_9FLAO]] Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place. Also tolerant to hybrid substrates containing C6-sulfate groups at the -4, +1, and +3 positions.<ref>PMID:15456406</ref> <ref>PMID:22778272</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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[[Category: Beta-agarase]] | [[Category: Beta-agarase]] | ||
[[Category: Zobellia galactanivorans]] | [[Category: Zobellia galactanivorans]] | ||
| - | [[Category: Allouch, J | + | [[Category: Allouch, J]] |
| - | [[Category: Barbeyron, T | + | [[Category: Barbeyron, T]] |
| - | [[Category: Czjzek, M | + | [[Category: Czjzek, M]] |
| - | [[Category: Helbert, W | + | [[Category: Helbert, W]] |
| - | [[Category: Henrissat, B | + | [[Category: Henrissat, B]] |
| - | [[Category: Jam, M | + | [[Category: Jam, M]] |
| - | [[Category: Kloareg, B | + | [[Category: Kloareg, B]] |
[[Category: 4-d-galactose linkage]] | [[Category: 4-d-galactose linkage]] | ||
[[Category: Agarose degradation]] | [[Category: Agarose degradation]] | ||
| - | [[Category: Beta-agarase]] | ||
[[Category: Cleavage of beta-1]] | [[Category: Cleavage of beta-1]] | ||
[[Category: Glycoside hydrolase family 16]] | [[Category: Glycoside hydrolase family 16]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
Revision as of 02:47, 25 December 2014
THE THREE-DIMENSIONAL STRUCTURE OF BETA-AGARASE B FROM ZOBELLIA GALACTANIVORANS
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