2b3x
From Proteopedia
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| - | [[Image:2b3x.gif|left|200px]] | + | [[Image:2b3x.gif|left|200px]] |
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| - | '''Structure of an orthorhombic crystal form of human cytosolic aconitase (IRP1)''' | + | {{Structure |
| + | |PDB= 2b3x |SIZE=350|CAPTION= <scene name='initialview01'>2b3x</scene>, resolution 2.540Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Aconitate_hydratase Aconitate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.3 4.2.1.3] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Structure of an orthorhombic crystal form of human cytosolic aconitase (IRP1)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2B3X is a [ | + | 2B3X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B3X OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of human iron regulatory protein 1 as cytosolic aconitase., Dupuy J, Volbeda A, Carpentier P, Darnault C, Moulis JM, Fontecilla-Camps JC, Structure. 2006 Jan;14(1):129-39. PMID:[http:// | + | Crystal structure of human iron regulatory protein 1 as cytosolic aconitase., Dupuy J, Volbeda A, Carpentier P, Darnault C, Moulis JM, Fontecilla-Camps JC, Structure. 2006 Jan;14(1):129-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16407072 16407072] |
[[Category: Aconitate hydratase]] | [[Category: Aconitate hydratase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: irp1 ire-irp1 aconitase activity]] | [[Category: irp1 ire-irp1 aconitase activity]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:57:17 2008'' |
Revision as of 13:57, 20 March 2008
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| , resolution 2.540Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | Aconitate hydratase, with EC number 4.2.1.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of an orthorhombic crystal form of human cytosolic aconitase (IRP1)
Overview
Iron regulatory proteins (IRPs) control the translation of proteins involved in iron uptake, storage and utilization by binding to specific noncoding sequences of the corresponding mRNAs known as iron-responsive elements (IREs). This strong interaction assures proper iron homeostasis in animal cells under iron shortage. Conversely, under iron-replete conditions, IRP1 binds a [4Fe-4S] cluster and functions as cytosolic aconitase. Regulation of the balance between the two IRP1 activities is complex, and it does not depend only on iron availability. Here, we report the crystal structure of human IRP1 in its aconitase form. Comparison with known structures of homologous enzymes reveals well-conserved folds and active site environments with significantly different surface shapes and charge distributions. The specific features of human IRP1 allow us to propose a tentative model of an IRP1-IRE complex that agrees with a range of previously obtained data.
About this Structure
2B3X is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human iron regulatory protein 1 as cytosolic aconitase., Dupuy J, Volbeda A, Carpentier P, Darnault C, Moulis JM, Fontecilla-Camps JC, Structure. 2006 Jan;14(1):129-39. PMID:16407072
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