2b4b
From Proteopedia
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| - | [[Image:2b4b.gif|left|200px]] | + | [[Image:2b4b.gif|left|200px]] |
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| - | '''SSAT+COA+BE-3-3-3, K6R mutant''' | + | {{Structure |
| + | |PDB= 2b4b |SIZE=350|CAPTION= <scene name='initialview01'>2b4b</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=COA:COENZYME+A'>COA</scene> and <scene name='pdbligand=B33:N-ETHYL-N-[3-(PROPYLAMINO)PROPYL]PROPANE-1,3-DIAMINE'>B33</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Diamine_N-acetyltransferase Diamine N-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.57 2.3.1.57] | ||
| + | |GENE= SAT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
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| + | '''SSAT+COA+BE-3-3-3, K6R mutant''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2B4B is a [ | + | 2B4B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B4B OCA]. |
==Reference== | ==Reference== | ||
| - | Structures of wild-type and mutant human spermidine/spermine N1-acetyltransferase, a potential therapeutic drug target., Bewley MC, Graziano V, Jiang J, Matz E, Studier FW, Pegg AE, Coleman CS, Flanagan JM, Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2063-8. Epub 2006 Feb 2. PMID:[http:// | + | Structures of wild-type and mutant human spermidine/spermine N1-acetyltransferase, a potential therapeutic drug target., Bewley MC, Graziano V, Jiang J, Matz E, Studier FW, Pegg AE, Coleman CS, Flanagan JM, Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2063-8. Epub 2006 Feb 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16455797 16455797] |
[[Category: Diamine N-acetyltransferase]] | [[Category: Diamine N-acetyltransferase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:57:24 2008'' |
Revision as of 13:57, 20 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | SAT (Homo sapiens) | ||||||
| Activity: | Diamine N-acetyltransferase, with EC number 2.3.1.57 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SSAT+COA+BE-3-3-3, K6R mutant
Contents |
Overview
Spermidine/spermine N1-acetyltransferase (SSAT) is a key enzyme in the control of polyamine levels in human cells, as acetylation of spermidine and spermine triggers export or degradation. Increased intracellular polyamine levels accompany several types of cancers as well as other human diseases, and compounds that affect the expression, activity, or stability of SSAT are being explored as potential therapeutic drugs. We have expressed human SSAT from the cloned cDNA in Escherichia coli and have determined high-resolution structures of wild-type and mutant SSAT, as the free dimer and in binary and ternary complexes with CoA, acetyl-CoA (AcCoA), spermine, and the inhibitor N1,N11bis-(ethyl)-norspermine (BE-3-3-3). These structures show details of binding sites for cofactor, substrates, and inhibitor and provide a framework to understand enzymatic activity, mutations, and the action of potential drugs. Two dimer conformations were observed: a symmetric form with two open surface channels capable of binding substrate or cofactor, and an asymmetric form in which only one of the surface channels appears capable of binding and acetylating polyamines. SSAT was found to self-acetylate lysine-26 in the presence of AcCoA and absence of substrate, a reaction apparently catalzyed by AcCoA bound in the second channel of the asymmetric dimer. These unexpected and intriguing complexities seem likely to have some as yet undefined role in regulating SSAT activity or stability as a part of polyamine homeostasis. Sequence signatures group SSAT with proteins that appear to have thialysine Nepsilon-acetyltransferase activity.
Disease
Known diseases associated with this structure: Keratosis follicularis spinulosa decalvans OMIM:[313020]
About this Structure
2B4B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structures of wild-type and mutant human spermidine/spermine N1-acetyltransferase, a potential therapeutic drug target., Bewley MC, Graziano V, Jiang J, Matz E, Studier FW, Pegg AE, Coleman CS, Flanagan JM, Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2063-8. Epub 2006 Feb 2. PMID:16455797
Page seeded by OCA on Thu Mar 20 15:57:24 2008
Categories: Diamine N-acetyltransferase | Homo sapiens | Single protein | Bewley, M C. | Coleman, C S. | Flanagan, J M. | Graziano, V. | Jiang, J S. | Matz, E. | NYSGXRC, New York Structural GenomiX Research Consortium. | Pegg, A P. | Studier, F W. | B33 | COA | New york structural genomix research consortium | Nysgxrc | Protein structure initiative | Psi | Structural genomic
