2b5a
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2b5a.gif|left|200px]] | + | [[Image:2b5a.gif|left|200px]] |
| - | + | ||
| - | '''C.BclI, Control Element of the BclI Restriction-Modification System''' | + | {{Structure |
| + | |PDB= 2b5a |SIZE=350|CAPTION= <scene name='initialview01'>2b5a</scene>, resolution 1.543Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ACY:ACETIC ACID'>ACY</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= bclIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1394 Bacillus caldolyticus]) | ||
| + | }} | ||
| + | |||
| + | '''C.BclI, Control Element of the BclI Restriction-Modification System''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2B5A is a [ | + | 2B5A is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_caldolyticus Bacillus caldolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site., Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK, Structure. 2005 Dec;13(12):1837-47. PMID:[http:// | + | Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site., Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK, Structure. 2005 Dec;13(12):1837-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16338412 16338412] |
[[Category: Bacillus caldolyticus]] | [[Category: Bacillus caldolyticus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 23: | Line 32: | ||
[[Category: helix-turn-helix motif]] | [[Category: helix-turn-helix motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:57:46 2008'' |
Revision as of 13:57, 20 March 2008
| |||||||
| , resolution 1.543Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | bclIC (Bacillus caldolyticus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
C.BclI, Control Element of the BclI Restriction-Modification System
Overview
Protection from DNA invasion is afforded by restriction-modification systems in many bacteria. The efficiency of protection depends crucially on the relative expression levels of restriction versus methytransferase genes. This regulation is provided by a controller protein, named C protein. Studies of the Bcll system in E. coli suggest that C.Bcll functions as a negative regulator for M.Bcll expression, implying that it plays a role in defense against foreign DNA during virus infection. C.Bcll binds (Kd = 14.3 nM) to a 2-fold symmetric C box DNA sequence that overlaps with the putative -35 promoter region upstream of the bcllM and bcllC genes. The C.Bcll fold comprises five alpha helices: two helices form a helix-turn-helix motif, and the remaining three helices form the extensive dimer interface. The C.Bcll-DNA model proposed suggests that DNA bending might play an important role in gene regulation, and that Glu27 and Asp31 in C.Bcll might function critically in the regulation.
About this Structure
2B5A is a Protein complex structure of sequences from Bacillus caldolyticus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site., Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK, Structure. 2005 Dec;13(12):1837-47. PMID:16338412
Page seeded by OCA on Thu Mar 20 15:57:46 2008
