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4k90
From Proteopedia
(Difference between revisions)
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| - | + | ==Extracellular metalloproteinase from Aspergillus== | |
| - | === | + | <StructureSection load='4k90' size='340' side='right' caption='[[4k90]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4k90]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus_af293 Aspergillus fumigatus af293] and [http://en.wikipedia.org/wiki/Zasmidium_angulare Zasmidium angulare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K90 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4K90 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=BO3:BORIC+ACID'>BO3</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mep, AFUA_8G07080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330879 Aspergillus fumigatus Af293]), mep, AFUA_8G07080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1194242 Zasmidium angulare])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k90 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k90 RCSB], [http://www.ebi.ac.uk/pdbsum/4k90 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/ELM_ASPFU ELM_ASPFU]] Secreted metalloproteinase that allows assimilation of proteinaceous substrates and probably acts as a virulence factor (By similarity). Catalyzes the hydrolysis of elastin. | [[http://www.uniprot.org/uniprot/ELM_ASPFU ELM_ASPFU]] Secreted metalloproteinase that allows assimilation of proteinaceous substrates and probably acts as a virulence factor (By similarity). Catalyzes the hydrolysis of elastin. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Fungalysins are secreted fungal peptidases with the ability to degrade the extracellular matrix proteins elastin and collagen and are thought to act as virulence factors in diseases caused by fungi. Fungalysins constitute a unique family among zinc-dependent peptidases that bears low sequence similarity to known bacterial peptidases of the thermolysin family. The crystal structure of the archetype of the fungalysin family, Aspergillus fumigatus metalloprotease (AfuMep), has been obtained for the first time. The 1.8 A resolution structure of AfuMep corresponds to that of an autoproteolyzed proenzyme with separate polypeptide chains corresponding to the N-terminal prodomain in a binary complex with the C-terminal zinc-bound catalytic domain. The prodomain consists of a tandem of cystatin-like folds whose C-terminal end is buried into the active-site cleft of the catalytic domain. The catalytic domain harbouring the key catalytic zinc ion and its ligands, two histidines and one glutamic acid, undergoes a conspicuous rearrangement of its N-terminal end during maturation. One key positively charged amino-acid residue and the C-terminal disulfide bridge appear to contribute to its structural-functional properties. Thus, structural, biophysical and biochemical analysis were combined to provide a deeper comprehension of the underlying properties of A. fumigatus fungalysin, serving as a framework for the as yet poorly known metallopeptidases from pathogenic fungi. | ||
| - | + | A functional and structural study of the major metalloprotease secreted by the pathogenic fungus Aspergillus fumigatus.,Fernandez D, Russi S, Vendrell J, Monod M, Pallares I Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):1946-57. doi:, 10.1107/S0907444913017642. Epub 2013 Sep 20. PMID:24100314<ref>PMID:24100314</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Aspergillus fumigatus af293]] | [[Category: Aspergillus fumigatus af293]] | ||
[[Category: Zasmidium angulare]] | [[Category: Zasmidium angulare]] | ||
| - | [[Category: Fernandez, D | + | [[Category: Fernandez, D]] |
| - | [[Category: Monod, M | + | [[Category: Monod, M]] |
| - | [[Category: Pallares, I | + | [[Category: Pallares, I]] |
| - | [[Category: Russi, S | + | [[Category: Russi, S]] |
| - | [[Category: Vendrell, J | + | [[Category: Vendrell, J]] |
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: M36 protease]] | [[Category: M36 protease]] | ||
Revision as of 02:57, 25 December 2014
Extracellular metalloproteinase from Aspergillus
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