2b63
From Proteopedia
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| - | [[Image:2b63.gif|left|200px]] | + | [[Image:2b63.gif|left|200px]] |
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| - | '''Complete RNA Polymerase II-RNA inhibitor complex''' | + | {{Structure |
| + | |PDB= 2b63 |SIZE=350|CAPTION= <scene name='initialview01'>2b63</scene>, resolution 3.800Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | ||
| + | |GENE= RPB4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), RPB7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Complete RNA Polymerase II-RNA inhibitor complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2B63 is a [ | + | 2B63 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B63 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs., Kettenberger H, Eisenfuhr A, Brueckner F, Theis M, Famulok M, Cramer P, Nat Struct Mol Biol. 2006 Jan;13(1):44-8. Epub 2005 Dec 11. PMID:[http:// | + | Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs., Kettenberger H, Eisenfuhr A, Brueckner F, Theis M, Famulok M, Cramer P, Nat Struct Mol Biol. 2006 Jan;13(1):44-8. Epub 2005 Dec 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16341226 16341226] |
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: rna polymerase ii]] | [[Category: rna polymerase ii]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:58:01 2008'' |
Revision as of 13:58, 20 March 2008
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| , resolution 3.800Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | RPB4 (Saccharomyces cerevisiae), RPB7 (Saccharomyces cerevisiae) | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Complete RNA Polymerase II-RNA inhibitor complex
Overview
The noncoding RNA B2 and the RNA aptamer FC bind RNA polymerase (Pol) II and inhibit messenger RNA transcription initiation, but not elongation. We report the crystal structure of FC(*), the central part of FC RNA, bound to Pol II. FC(*) RNA forms a double stem-loop structure in the Pol II active center cleft. B2 RNA may bind similarly, as it competes with FC(*) RNA for Pol II interaction. Both RNA inhibitors apparently prevent the downstream DNA duplex and the template single strand from entering the cleft after DNA melting and thus interfere with open-complex formation. Elongation is not inhibited, as nucleic acids prebound in the cleft would exclude the RNA inhibitors. The structure also indicates that A-form RNA could interact with Pol II similarly to a B-form DNA promoter, as suggested for the bacterial transcription inhibitor 6S RNA.
About this Structure
2B63 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure of an RNA polymerase II-RNA inhibitor complex elucidates transcription regulation by noncoding RNAs., Kettenberger H, Eisenfuhr A, Brueckner F, Theis M, Famulok M, Cramer P, Nat Struct Mol Biol. 2006 Jan;13(1):44-8. Epub 2005 Dec 11. PMID:16341226
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