2b7x
From Proteopedia
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| - | [[Image:2b7x.gif|left|200px]] | + | [[Image:2b7x.gif|left|200px]] |
| - | + | ||
| - | '''Sequential reorganization of beta-sheet topology by insertion of a single strand''' | + | {{Structure |
| + | |PDB= 2b7x |SIZE=350|CAPTION= <scene name='initialview01'>2b7x</scene>, resolution 3.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | ||
| + | |GENE= E ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T4]) | ||
| + | }} | ||
| + | |||
| + | '''Sequential reorganization of beta-sheet topology by insertion of a single strand''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2B7X is a [ | + | 2B7X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B7X OCA]. |
==Reference== | ==Reference== | ||
| - | Sequential reorganization of beta-sheet topology by insertion of a single strand., Sagermann M, Baase WA, Matthews BW, Protein Sci. 2006 May;15(5):1085-92. Epub 2006 Apr 5. PMID:[http:// | + | Sequential reorganization of beta-sheet topology by insertion of a single strand., Sagermann M, Baase WA, Matthews BW, Protein Sci. 2006 May;15(5):1085-92. Epub 2006 Apr 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16597830 16597830] |
[[Category: Bacteriophage t4]] | [[Category: Bacteriophage t4]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
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[[Category: protein design]] | [[Category: protein design]] | ||
[[Category: sequence duplication]] | [[Category: sequence duplication]] | ||
| - | [[Category: structural | + | [[Category: structural switch]] |
[[Category: tandem repeat]] | [[Category: tandem repeat]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:58:37 2008'' |
Revision as of 13:58, 20 March 2008
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| , resolution 3.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | E (Bacteriophage T4) | ||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Sequential reorganization of beta-sheet topology by insertion of a single strand
Overview
Insertions, duplications, and deletions of sequence segments are thought to be major evolutionary mechanisms that increase the structural and functional diversity of proteins. Alternative splicing, for example, is an intracellular editing mechanism that is thought to generate isoforms for 30%-50% of all human genes. Whereas the inserted sequences usually display only minor structural rearrangements at the insertion site, recent observations indicate that they may also cause more dramatic structural displacements of adjacent structures. In the present study we test how artificially inserted sequences change the structure of the beta-sheet region in T4 lysozyme. Copies of two different beta-strands were inserted into two different loops of the beta-sheet, and the structures were determined. Not surprisingly, one insert "loops out" at its insertion site and forms a new small beta-hairpin structure. Unexpectedly, however, the second insertion leads to displacement of adjacent strands and a sequential reorganization of the beta-sheet topology. Even though the insertions were performed at two different sites, looping out occurred at the C-terminal end of the same beta-strand. Reasons as to why a non-native sequence would be recruited to replace that which occurs in the native protein are discussed. Our results illustrate how sequence insertions can facilitate protein evolution through both local and nonlocal changes in structure.
About this Structure
2B7X is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.
Reference
Sequential reorganization of beta-sheet topology by insertion of a single strand., Sagermann M, Baase WA, Matthews BW, Protein Sci. 2006 May;15(5):1085-92. Epub 2006 Apr 5. PMID:16597830
Page seeded by OCA on Thu Mar 20 15:58:37 2008
