2h7o

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(Difference between revisions)

Revision as of 03:21, 25 December 2014

Crystal structure of the Rho-GTPase binding domain of YpkA

Structural highlights

2h7o is a 1 chain structure with sequence from "bacillus_pseudotuberkulosis"_(sic)_pfeiffer_1889 "bacillus pseudotuberkulosis" (sic) pfeiffer 1889. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	ypkA ("Bacillus pseudotuberkulosis" (sic) Pfeiffer 1889)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[YPKA_YERPS] Acts as a virulence determinant.^[1] ^[2]

Publication Abstract from PubMed

Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.

Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors.,Prehna G, Ivanov MI, Bliska JB, Stebbins CE Cell. 2006 Sep 8;126(5):869-80. PMID:16959567^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Juris SJ, Rudolph AE, Huddler D, Orth K, Dixon JE. A distinctive role for the Yersinia protein kinase: actin binding, kinase activation, and cytoskeleton disruption. Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9431-6. PMID:10920208 doi:http://dx.doi.org/10.1073/pnas.170281997
↑ Navarro L, Koller A, Nordfelth R, Wolf-Watz H, Taylor S, Dixon JE. Identification of a molecular target for the Yersinia protein kinase A. Mol Cell. 2007 May 25;26(4):465-77. PMID:17531806 doi:http://dx.doi.org/S1097-2765(07)00285-7
↑ Prehna G, Ivanov MI, Bliska JB, Stebbins CE. Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors. Cell. 2006 Sep 8;126(5):869-80. PMID:16959567 doi:10.1016/j.cell.2006.06.056

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2h7o"

@@ Line 6: / Line 6: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2h7o RCSB], [http://www.ebi.ac.uk/pdbsum/2h7o PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/YPKA_YERPS YPKA_YERPS]] Acts as a virulence determinant.<ref>PMID:10920208</ref> <ref>PMID:17531806</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

2h7o

From Proteopedia

Revision as of 03:21, 25 December 2014

Crystal structure of the Rho-GTPase binding domain of YpkA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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