| Structural highlights
Function
[SG101_ARATH] Acyl hydrolase that triggers the leaf senescence onset. Can use triolein as substrate to produce oleic acids.[1] [2] [3] [4] Involved in the EDS1-dependent intrinsic and indispensable resistance signaling pathway; together with PAD4, required for programmed cell death triggered by RPS4 in response to avirulent pathogens (e.g. Pseudomonas syringae pv. tomato strain DC3000 and Hyaloperonospora parasitica isolates CALA2 and EMWA1) and in restricting the growth of virulent pathogens (e.g. H. parasitica isolates NOCO2 and P.syringae pv. tomato strain DC3000 avrRps4). Regulates the nuclear localization of EDS1. Essential for the RPP8/HRT-mediated resistance to the turnip crinkle virus (TCV). Involved in the post-invasion resistance to Phakopsora pachyrhizi in the mesophyll.[5] [6] [7] [8]
Publication Abstract from PubMed
Biotrophic plant pathogens encounter a postinfection basal resistance layer controlled by the lipase-like protein enhanced disease susceptibility 1 (EDS1) and its sequence-related interaction partners, senescence-associated gene 101 (SAG101) and phytoalexin deficient 4 (PAD4). Maintainance of separate EDS1 family member clades through angiosperm evolution suggests distinct functional attributes. We report the Arabidopsis EDS1-SAG101 heterodimer crystal structure with juxtaposed N-terminal alpha/beta hydrolase and C-terminal alpha-helical EP domains aligned via a large conserved interface. Mutational analysis of the EDS1-SAG101 heterodimer and a derived EDS1-PAD4 structural model shows that EDS1 signals within mutually exclusive heterocomplexes. Although there is evolutionary conservation of alpha/beta hydrolase topology in all three proteins, a noncatalytic resistance mechanism is indicated. Instead, the respective N-terminal domains appear to facilitate binding of the essential EP domains to create novel interaction surfaces on the heterodimer. Transitions between distinct functional EDS1 heterodimers might explain the central importance and versatility of this regulatory node in plant immunity.
Structural basis for signaling by exclusive EDS1 heteromeric complexes with SAG101 or PAD4 in plant innate immunity.,Wagner S, Stuttmann J, Rietz S, Guerois R, Brunstein E, Bautor J, Niefind K, Parker JE Cell Host Microbe. 2013 Dec 11;14(6):619-30. doi: 10.1016/j.chom.2013.11.006. PMID:24331460[9]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ He Y, Gan S. A gene encoding an acyl hydrolase is involved in leaf senescence in Arabidopsis. Plant Cell. 2002 Apr;14(4):805-15. PMID:11971136
- ↑ Feys BJ, Wiermer M, Bhat RA, Moisan LJ, Medina-Escobar N, Neu C, Cabral A, Parker JE. Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity. Plant Cell. 2005 Sep;17(9):2601-13. Epub 2005 Jul 22. PMID:16040633 doi:http://dx.doi.org/tpc.105.033910
- ↑ Zhu S, Jeong RD, Venugopal SC, Lapchyk L, Navarre D, Kachroo A, Kachroo P. SAG101 forms a ternary complex with EDS1 and PAD4 and is required for resistance signaling against turnip crinkle virus. PLoS Pathog. 2011 Nov;7(11):e1002318. doi: 10.1371/journal.ppat.1002318. Epub, 2011 Nov 3. PMID:22072959 doi:http://dx.doi.org/10.1371/journal.ppat.1002318
- ↑ Langenbach C, Campe R, Schaffrath U, Goellner K, Conrath U. UDP-glucosyltransferase UGT84A2/BRT1 is required for Arabidopsis nonhost resistance to the Asian soybean rust pathogen Phakopsora pachyrhizi. New Phytol. 2013 Apr;198(2):536-45. doi: 10.1111/nph.12155. Epub 2013 Jan 29. PMID:23356583 doi:http://dx.doi.org/10.1111/nph.12155
- ↑ He Y, Gan S. A gene encoding an acyl hydrolase is involved in leaf senescence in Arabidopsis. Plant Cell. 2002 Apr;14(4):805-15. PMID:11971136
- ↑ Feys BJ, Wiermer M, Bhat RA, Moisan LJ, Medina-Escobar N, Neu C, Cabral A, Parker JE. Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity. Plant Cell. 2005 Sep;17(9):2601-13. Epub 2005 Jul 22. PMID:16040633 doi:http://dx.doi.org/tpc.105.033910
- ↑ Zhu S, Jeong RD, Venugopal SC, Lapchyk L, Navarre D, Kachroo A, Kachroo P. SAG101 forms a ternary complex with EDS1 and PAD4 and is required for resistance signaling against turnip crinkle virus. PLoS Pathog. 2011 Nov;7(11):e1002318. doi: 10.1371/journal.ppat.1002318. Epub, 2011 Nov 3. PMID:22072959 doi:http://dx.doi.org/10.1371/journal.ppat.1002318
- ↑ Langenbach C, Campe R, Schaffrath U, Goellner K, Conrath U. UDP-glucosyltransferase UGT84A2/BRT1 is required for Arabidopsis nonhost resistance to the Asian soybean rust pathogen Phakopsora pachyrhizi. New Phytol. 2013 Apr;198(2):536-45. doi: 10.1111/nph.12155. Epub 2013 Jan 29. PMID:23356583 doi:http://dx.doi.org/10.1111/nph.12155
- ↑ Wagner S, Stuttmann J, Rietz S, Guerois R, Brunstein E, Bautor J, Niefind K, Parker JE. Structural basis for signaling by exclusive EDS1 heteromeric complexes with SAG101 or PAD4 in plant innate immunity. Cell Host Microbe. 2013 Dec 11;14(6):619-30. doi: 10.1016/j.chom.2013.11.006. PMID:24331460 doi:http://dx.doi.org/10.1016/j.chom.2013.11.006
|
