2bdb

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[[Image:2bdb.gif|left|200px]]<br /><applet load="2bdb" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2bdb.gif|left|200px]]
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caption="2bdb, resolution 1.700&Aring;" />
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'''Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0'''<br />
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{{Structure
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|PDB= 2bdb |SIZE=350|CAPTION= <scene name='initialview01'>2bdb</scene>, resolution 1.700&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=ALA:ALANINE'>ALA</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36]
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|GENE=
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}}
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'''Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2BDB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ALA:'>ALA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDB OCA].
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2BDB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDB OCA].
==Reference==
==Reference==
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Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9187653 9187653]
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Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9187653 9187653]
[[Category: Pancreatic elastase]]
[[Category: Pancreatic elastase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: serine proteinase]]
[[Category: serine proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:36:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:00:24 2008''

Revision as of 14:00, 20 March 2008

Image:2bdb.gif


PDB ID 2bdb

Drag the structure with the mouse to rotate
, resolution 1.700Å
Ligands: , and
Activity: Pancreatic elastase, with EC number 3.4.21.36
Coordinates: save as pdb, mmCIF, xml



Porcine pancreatic elastase complexed with Asn-Pro-Ile and Ala-Ala at pH 5.0


Overview

Mass spectrometric screening reveals that an unmodified natural heptapeptide--human beta-casomorphin-7, an internal sequence of human beta-casein that possesses opioid-like activity--reacts with porcine pancreatic elastase to form an unusually stable acyl-enzyme complex at low pH. X-ray crystallographic analysis (to 1.9 A resolution) at pH 5 shows continuous electron density linking the C-terminal isoleucine of beta-casomorphin-7 to Ser 195 through an ester bond. The structure reveals a well defined water molecule (Wat 317), equidistant between the carbon of the ester carbonyl and N epsilon 2 of His 57. Deprotonation of Wat 317 will produce a hydroxide ion positioned to attack the ester carbonyl through the favoured Burgi-Dunitz trajectory.

About this Structure

2BDB is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

Reference

Structure of a specific acyl-enzyme complex formed between beta-casomorphin-7 and porcine pancreatic elastase., Wilmouth RC, Clifton IJ, Robinson CV, Roach PL, Aplin RT, Westwood NJ, Hajdu J, Schofield CJ, Nat Struct Biol. 1997 Jun;4(6):456-62. PMID:9187653

Page seeded by OCA on Thu Mar 20 16:00:24 2008

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