2be6
From Proteopedia
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| - | [[Image:2be6.gif|left|200px]] | + | [[Image:2be6.gif|left|200px]] |
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| - | '''2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex''' | + | {{Structure |
| + | |PDB= 2be6 |SIZE=350|CAPTION= <scene name='initialview01'>2be6</scene>, resolution 2.000Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CALM2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CACNA1C, CACH2, CACN2, CACNL1A1, CCHL1A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==Disease== | ==Disease== | ||
| - | Known diseases associated with this structure: Timothy syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=114205 114205]] | + | Known diseases associated with this structure: Brugada syndrome 3 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=114205 114205]], Timothy syndrome OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=114205 114205]] |
==About this Structure== | ==About this Structure== | ||
| - | 2BE6 is a [ | + | 2BE6 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BE6 OCA]. |
==Reference== | ==Reference== | ||
| - | Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex., Van Petegem F, Chatelain FC, Minor DL Jr, Nat Struct Mol Biol. 2005 Dec;12(12):1108-15. Epub 2005 Nov 20. PMID:[http:// | + | Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex., Van Petegem F, Chatelain FC, Minor DL Jr, Nat Struct Mol Biol. 2005 Dec;12(12):1108-15. Epub 2005 Nov 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16299511 16299511] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: voltage-gated]] | [[Category: voltage-gated]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:00:45 2008'' |
Revision as of 14:00, 20 March 2008
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| , resolution 2.000Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | CALM2 (Homo sapiens), CACNA1C, CACH2, CACN2, CACNL1A1, CCHL1A1 (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
2.0 A crystal structure of the CaV1.2 IQ domain-Ca/CaM complex
Contents |
Overview
Changes in activity-dependent calcium flux through voltage-gated calcium channels (Ca(V)s) drive two self-regulatory calcium-dependent feedback processes that require interaction between Ca(2+)/calmodulin (Ca(2+)/CaM) and a Ca(V) channel consensus isoleucine-glutamine (IQ) motif: calcium-dependent inactivation (CDI) and calcium-dependent facilitation (CDF). Here, we report the high-resolution structure of the Ca(2+)/CaM-Ca(V)1.2 IQ domain complex. The IQ domain engages hydrophobic pockets in the N-terminal and C-terminal Ca(2+)/CaM lobes through sets of conserved 'aromatic anchors.' Ca(2+)/N lobe adopts two conformations that suggest inherent conformational plasticity at the Ca(2+)/N lobe-IQ domain interface. Titration calorimetry experiments reveal competition between the lobes for IQ domain sites. Electrophysiological examination of Ca(2+)/N lobe aromatic anchors uncovers their role in Ca(V)1.2 CDF. Together, our data suggest that Ca(V) subtype differences in CDI and CDF are tuned by changes in IQ domain anchoring positions and establish a framework for understanding CaM lobe-specific regulation of Ca(V)s.
Disease
Known diseases associated with this structure: Brugada syndrome 3 OMIM:[114205], Timothy syndrome OMIM:[114205]
About this Structure
2BE6 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Insights into voltage-gated calcium channel regulation from the structure of the CaV1.2 IQ domain-Ca2+/calmodulin complex., Van Petegem F, Chatelain FC, Minor DL Jr, Nat Struct Mol Biol. 2005 Dec;12(12):1108-15. Epub 2005 Nov 20. PMID:16299511
Page seeded by OCA on Thu Mar 20 16:00:45 2008
