3rix

From Proteopedia

(Difference between revisions)

Revision as of 04:11, 25 December 2014

1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex

Structural highlights

3rix is a 1 chain structure with sequence from Photinus pyralis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Activity:	Photinus-luciferin 4-monooxygenase (ATP-hydrolyzing), with EC number 1.13.12.7
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[LUCI_PHOPY] Produces green light with a wavelength of 562 nm.

Publication Abstract from PubMed

The chemical diversity of nature has tremendous potential for the discovery of molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, and macro- and microorganisms has curtailed their use in lead discovery. Here, we describe a process for leveraging the concentration-response curves obtained from quantitative HTS to improve the initial selection of "actives" from a library of partially fractionated natural product extracts derived from marine actinomycetes and fungi. By using pharmacological activity, the first-pass CRC paradigm improves the probability that labor-intensive subsequent steps of reculturing, extraction, and bioassay-guided isolation of active component(s) target the most promising strains and growth conditions. We illustrate how this process identified a family of fungal metabolites as potent inhibitors of firefly luciferase, subsequently resolved in molecular detail by X-ray crystallography.

Titration-based screening for evaluation of natural product extracts: identification of an aspulvinone family of luciferase inhibitors.,Cruz PG, Auld DS, Schultz PJ, Lovell S, Battaile KP, MacArthur R, Shen M, Tamayo-Castillo G, Inglese J, Sherman DH Chem Biol. 2011 Nov 23;18(11):1442-52. PMID:22118678^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cruz PG, Auld DS, Schultz PJ, Lovell S, Battaile KP, MacArthur R, Shen M, Tamayo-Castillo G, Inglese J, Sherman DH. Titration-based screening for evaluation of natural product extracts: identification of an aspulvinone family of luciferase inhibitors. Chem Biol. 2011 Nov 23;18(11):1442-52. PMID:22118678 doi:10.1016/j.chembiol.2011.08.011

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3rix"

@@ Line 7: / Line 7: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rix OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rix RCSB], [http://www.ebi.ac.uk/pdbsum/3rix PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/LUCI_PHOPY LUCI_PHOPY]] Produces green light with a wavelength of 562 nm.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

3rix

From Proteopedia

Revision as of 04:11, 25 December 2014

1.7A resolution structure of a firefly luciferase-Aspulvinone J inhibitor complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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