2y7h
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y7h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y7h RCSB], [http://www.ebi.ac.uk/pdbsum/2y7h PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y7h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y7h RCSB], [http://www.ebi.ac.uk/pdbsum/2y7h PDBsum]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/T1MK_ECOLI T1MK_ECOLI]] The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. The EcoKI enzyme recognizes 5'-AACN(6)GTGC-3'. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Type I site-specific deoxyribonuclease]] | [[Category: Type I site-specific deoxyribonuclease]] | ||
| - | [[Category: Bujnicki, J M | + | [[Category: Bujnicki, J M]] |
| - | [[Category: Cooper, L P | + | [[Category: Cooper, L P]] |
| - | [[Category: Dryden, D T.F | + | [[Category: Dryden, D T.F]] |
| - | [[Category: Kennaway, C K | + | [[Category: Kennaway, C K]] |
| - | [[Category: Obarska-Kosinska, A | + | [[Category: Obarska-Kosinska, A]] |
| - | [[Category: Trinick, J | + | [[Category: Trinick, J]] |
| - | [[Category: Tuszynska, I | + | [[Category: Tuszynska, I]] |
| - | [[Category: White, J H | + | [[Category: White, J H]] |
[[Category: Transferase-dna complex]] | [[Category: Transferase-dna complex]] | ||
Revision as of 04:17, 25 December 2014
Atomic model of the DNA-bound methylase complex from the Type I restriction-modification enzyme EcoKI (M2S1). Based on fitting into EM map 1534.
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