2xjx
From Proteopedia
(Difference between revisions)
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| - | + | ==Structure of HSP90 with small molecule inhibitor bound== | |
| - | + | <StructureSection load='2xjx' size='340' side='right' caption='[[2xjx]], [[Resolution|resolution]] 1.66Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[2xjx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XJX FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XJX:(2,4-DIHYDROXY-5-PROPAN-2-YL-PHENYL)-[5-[(4-METHYLPIPERAZIN-1-YL)METHYL]ISOINDOL-2-YL]METHANONE'>XJX</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2cdd|2cdd]], [[1yes|1yes]], [[1uy9|1uy9]], [[1byq|1byq]], [[2bsm|2bsm]], [[1osf|1osf]], [[2wi3|2wi3]], [[1uy8|1uy8]], [[2bug|2bug]], [[2wi4|2wi4]], [[2uwd|2uwd]], [[2xhx|2xhx]], [[2wi7|2wi7]], [[2bt0|2bt0]], [[1yer|1yer]], [[2xdu|2xdu]], [[1uyg|1uyg]], [[2bz5|2bz5]], [[2ccu|2ccu]], [[2xds|2xds]], [[2xdx|2xdx]], [[2ccs|2ccs]], [[1uyf|1uyf]], [[1yc3|1yc3]], [[1uyi|1uyi]], [[1uyd|1uyd]], [[2byi|2byi]], [[2xdl|2xdl]], [[2wi2|2wi2]], [[1uy6|1uy6]], [[2vci|2vci]], [[2wi1|2wi1]], [[2vcj|2vcj]], [[1yc4|1yc4]], [[2c2l|2c2l]], [[2fwz|2fwz]], [[2xdk|2xdk]], [[1uyh|1uyh]], [[2xjg|2xjg]], [[1uyk|1uyk]], [[2cct|2cct]], [[2fwy|2fwy]], [[1uyl|1uyl]], [[1uye|1uye]], [[2wi6|2wi6]], [[2xab|2xab]], [[1yc1|1yc1]], [[2xhr|2xhr]], [[1uyc|1uyc]], [[2xht|2xht]], [[1uy7|1uy7]], [[1yet|1yet]], [[2jjc|2jjc]], [[2byh|2byh]], [[2wi5|2wi5]], [[2xjj|2xjj]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xjx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xjx RCSB], [http://www.ebi.ac.uk/pdbsum/2xjx PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/HS90A_HUMAN HS90A_HUMAN]] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.<ref>PMID:15937123</ref> <ref>PMID:11274138</ref> | [[http://www.uniprot.org/uniprot/HS90A_HUMAN HS90A_HUMAN]] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.<ref>PMID:15937123</ref> <ref>PMID:11274138</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xj/2xjx_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Inhibitors of the molecular chaperone heat shock protein 90 (Hsp90) are currently generating significant interest in clinical development as potential treatments for cancer. In a preceding publication (DOI: 10.1021/jm100059d ) we describe Astex's approach to screening fragments against Hsp90 and the subsequent optimization of two hits into leads with inhibitory activities in the low nanomolar range. This paper describes the structure guided optimization of the 2,4-dihydroxybenzamide lead molecule 1 and details some of the drug discovery strategies employed in the identification of AT13387 (35), which has progressed through preclinical development and is currently being tested in man. | ||
| - | + | Discovery of (2,4-Dihydroxy-5-isopropylphenyl)-[5-(4-methylpiperazin-1-ylmethyl)-1,3-di hydroisoindol-2-yl]methanone (AT13387), a Novel Inhibitor of the Molecular Chaperone Hsp90 by Fragment Based Drug Design.,Woodhead AJ, Angove H, Carr MG, Chessari G, Congreve M, Coyle JE, Cosme J, Graham B, Day PJ, Downham R, Fazal L, Feltell R, Figueroa E, Frederickson M, Lewis J, McMenamin R, Murray CW, O'Brien MA, Parra L, Patel S, Phillips T, Rees DC, Rich S, Smith DM, Trewartha G, Vinkovic M, Williams B, Woolford AJ J Med Chem. 2010 Jul 28. PMID:20662534<ref>PMID:20662534</ref> | |
| - | [ | + | |
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Heat Shock Proteins|Heat Shock Proteins]] | *[[Heat Shock Proteins|Heat Shock Proteins]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: Callaghan, O | + | [[Category: Callaghan, O]] |
| - | [[Category: Carr, M G | + | [[Category: Carr, M G]] |
| - | [[Category: Chessari, G | + | [[Category: Chessari, G]] |
| - | [[Category: Congreve, M | + | [[Category: Congreve, M]] |
| - | [[Category: Cowan, S | + | [[Category: Cowan, S]] |
| - | [[Category: Coyle, J E | + | [[Category: Coyle, J E]] |
| - | [[Category: Downham, R | + | [[Category: Downham, R]] |
| - | [[Category: Figueroa, E | + | [[Category: Figueroa, E]] |
| - | [[Category: Frederickson, M | + | [[Category: Frederickson, M]] |
| - | [[Category: Graham, B | + | [[Category: Graham, B]] |
| - | [[Category: McMenamin, R | + | [[Category: McMenamin, R]] |
| - | [[Category: Murray, C W | + | [[Category: Murray, C W]] |
| - | [[Category: OBrien, M A | + | [[Category: OBrien, M A]] |
| - | [[Category: Patel, S | + | [[Category: Patel, S]] |
| - | [[Category: Phillips, T R | + | [[Category: Phillips, T R]] |
| - | [[Category: Williams, G | + | [[Category: Williams, G]] |
| - | [[Category: Woodhead, A J | + | [[Category: Woodhead, A J]] |
| - | [[Category: Woolford, A J.A | + | [[Category: Woolford, A J.A]] |
[[Category: Atp binding domain]] | [[Category: Atp binding domain]] | ||
[[Category: Chaperone]] | [[Category: Chaperone]] | ||
[[Category: Stress response]] | [[Category: Stress response]] | ||
Revision as of 04:17, 25 December 2014
Structure of HSP90 with small molecule inhibitor bound
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Categories: Homo sapiens | Callaghan, O | Carr, M G | Chessari, G | Congreve, M | Cowan, S | Coyle, J E | Downham, R | Figueroa, E | Frederickson, M | Graham, B | McMenamin, R | Murray, C W | OBrien, M A | Patel, S | Phillips, T R | Williams, G | Woodhead, A J | Woolford, A J.A | Atp binding domain | Chaperone | Stress response
