2bkj

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[[Image:2bkj.jpg|left|200px]]<br /><applet load="2bkj" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2bkj.jpg|left|200px]]
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caption="2bkj, resolution 2.08&Aring;" />
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'''NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI COMPLEXED WITH NAD+'''<br />
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{{Structure
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|PDB= 2bkj |SIZE=350|CAPTION= <scene name='initialview01'>2bkj</scene>, resolution 2.08&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene> and <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/FMN_reductase FMN reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.29 1.5.1.29]
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|GENE=
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}}
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'''NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI COMPLEXED WITH NAD+'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2BKJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi] with <scene name='pdbligand=FMN:'>FMN</scene> and <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/FMN_reductase FMN reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.29 1.5.1.29] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKJ OCA].
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2BKJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKJ OCA].
==Reference==
==Reference==
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Unusual folded conformation of nicotinamide adenine dinucleotide bound to flavin reductase P., Tanner JJ, Tu SC, Barbour LJ, Barnes CL, Krause KL, Protein Sci. 1999 Sep;8(9):1725-32. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10493573 10493573]
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Unusual folded conformation of nicotinamide adenine dinucleotide bound to flavin reductase P., Tanner JJ, Tu SC, Barbour LJ, Barnes CL, Krause KL, Protein Sci. 1999 Sep;8(9):1725-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10493573 10493573]
[[Category: FMN reductase]]
[[Category: FMN reductase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:38:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:03:08 2008''

Revision as of 14:03, 20 March 2008

Image:2bkj.jpg


PDB ID 2bkj

Drag the structure with the mouse to rotate
, resolution 2.08Å
Ligands: and
Activity: FMN reductase, with EC number 1.5.1.29
Coordinates: save as pdb, mmCIF, xml



NADPH:FMN OXIDOREDUCTASE FROM VIBRIO HARVEYI COMPLEXED WITH NAD+


Overview

The 2.1 A resolution crystal structure of flavin reductase P with the inhibitor nicotinamide adenine dinucleotide (NAD) bound in the active site has been determined. NAD adopts a novel, folded conformation in which the nicotinamide and adenine rings stack in parallel with an inter-ring distance of 3.6 A. The pyrophosphate binds next to the flavin cofactor isoalloxazine, while the stacked nicotinamide/adenine moiety faces away from the flavin. The observed NAD conformation is quite different from the extended conformations observed in other enzyme/NAD(P) structures; however, it resembles the conformation proposed for NAD in solution. The flavin reductase P/NAD structure provides new information about the conformational diversity of NAD, which is important for understanding catalysis. This structure offers the first crystallographic evidence of a folded NAD with ring stacking, and it is the first enzyme structure containing an FMN cofactor interacting with NAD(P). Analysis of the structure suggests a possible dynamic mechanism underlying NADPH substrate specificity and product release that involves unfolding and folding of NADP(H).

About this Structure

2BKJ is a Single protein structure of sequence from Vibrio harveyi. Full crystallographic information is available from OCA.

Reference

Unusual folded conformation of nicotinamide adenine dinucleotide bound to flavin reductase P., Tanner JJ, Tu SC, Barbour LJ, Barnes CL, Krause KL, Protein Sci. 1999 Sep;8(9):1725-32. PMID:10493573

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