3iky
From Proteopedia
(Difference between revisions)
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| - | + | ==Structural model of ParM filament in the open state by cryo-EM== | |
| - | + | <StructureSection load='3iky' size='340' side='right' caption='[[3iky]], [[Resolution|resolution]] 18.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3iky]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IKY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IKY FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3iku|3iku]]</td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">parM, stbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iky OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iky RCSB], [http://www.ebi.ac.uk/pdbsum/3iky PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/PARM_ECOLX PARM_ECOLX]] Involved in the control of plasmid partition. Required for the accurate segregation of the plasmid. | [[http://www.uniprot.org/uniprot/PARM_ECOLX PARM_ECOLX]] Involved in the control of plasmid partition. Required for the accurate segregation of the plasmid. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ik/3iky_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Segregation of the R1 plasmid in bacteria relies on ParM, an actin homolog that segregates plasmids by switching between cycles of polymerization and depolymerization. We find similar polymerization kinetics and stability in the presence of either ATP or GTP and a 10-fold affinity preference for ATP over GTP. We used electron cryo-microscopy to evaluate the heterogeneity within ParM filaments. In addition to variable twist, ParM has variable axial rise, and both parameters are coupled. Subunits in the same ParM filaments can exist in two different structural states, with the nucleotide-binding cleft closed or open, and the bound nucleotide biases the distribution of states. The interface between protomers is different between these states, and in neither state is it similar to F-actin. Our results suggest that the closed state of the cleft is required but not sufficient for ParM polymerization, and provide a structural basis for the dynamic instability of ParM filaments. | ||
| - | + | Structural polymorphism of the ParM filament and dynamic instability.,Galkin VE, Orlova A, Rivera C, Mullins RD, Egelman EH Structure. 2009 Sep 9;17(9):1253-64. PMID:19748346<ref>PMID:19748346</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Bacillus coli migula 1895]] | [[Category: Bacillus coli migula 1895]] | ||
| - | [[Category: Egelman, E H | + | [[Category: Egelman, E H]] |
| - | [[Category: Galkin, V E | + | [[Category: Galkin, V E]] |
| - | [[Category: Mullins, R D | + | [[Category: Mullins, R D]] |
| - | [[Category: Orlova, A | + | [[Category: Orlova, A]] |
| - | [[Category: Rivera, C | + | [[Category: Rivera, C]] |
[[Category: Polymorphic protein polymer]] | [[Category: Polymorphic protein polymer]] | ||
[[Category: Structural protein]] | [[Category: Structural protein]] | ||
Revision as of 04:40, 25 December 2014
Structural model of ParM filament in the open state by cryo-EM
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