2bl0
From Proteopedia
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| - | [[Image:2bl0.gif|left|200px]] | + | [[Image:2bl0.gif|left|200px]] |
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| - | '''PHYSARUM POLYCEPHALUM MYOSIN II REGULATORY DOMAIN''' | + | {{Structure |
| + | |PDB= 2bl0 |SIZE=350|CAPTION= <scene name='initialview01'>2bl0</scene>, resolution 1.75Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Ca+Binding+Site+For+Chain+C'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.4.1 Transferred entry: 3.6.4.1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.32 3.6.1.32] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''PHYSARUM POLYCEPHALUM MYOSIN II REGULATORY DOMAIN''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BL0 is a [ | + | 2BL0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BL0 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural evidence for non-canonical binding of Ca2+ to a canonical EF-hand of a conventional myosin., Debreczeni JE, Farkas L, Harmat V, Hetenyi C, Hajdu I, Zavodszky P, Kohama K, Nyitray L, J Biol Chem. 2005 Dec 16;280(50):41458-64. Epub 2005 Oct 13. PMID:[http:// | + | Structural evidence for non-canonical binding of Ca2+ to a canonical EF-hand of a conventional myosin., Debreczeni JE, Farkas L, Harmat V, Hetenyi C, Hajdu I, Zavodszky P, Kohama K, Nyitray L, J Biol Chem. 2005 Dec 16;280(50):41458-64. Epub 2005 Oct 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16227209 16227209] |
[[Category: Physarum polycephalum]] | [[Category: Physarum polycephalum]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: slime mould]] | [[Category: slime mould]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:03:19 2008'' |
Revision as of 14:03, 20 March 2008
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| , resolution 1.75Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Transferred entry: 3.6.4.1, with EC number 3.6.1.32 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PHYSARUM POLYCEPHALUM MYOSIN II REGULATORY DOMAIN
Overview
We have previously identified a single inhibitory Ca2+-binding site in the first EF-hand of the essential light chain of Physarum conventional myosin (Farkas, L., Malnasi-Csizmadia, A., Nakamura, A., Kohama, K., and Nyitray, L. (2003) J. Biol. Chem. 278, 27399-27405). As a general rule, conformation of the EF-hand-containing domains in the calmodulin family is "closed" in the absence and "open" in the presence of bound cations; a notable exception is the unusual Ca2+-bound closed domain in the essential light chain of the Ca2+-activated scallop muscle myosin. Here we have reported the 1.8 A resolution structure of the regulatory domain (RD) of Physarum myosin II in which Ca2+ is bound to a canonical EF-hand that is also in a closed state. The 12th position of the EF-hand loop, which normally provides a bidentate ligand for Ca2+ in the open state, is too far in the structure to participate in coordination of the ion. The structure includes a second Ca2+ that only mediates crystal contacts. To reveal the mechanism behind the regulatory effect of Ca2+, we compared conformational flexibilities of the liganded and unliganded RD. Our working hypothesis, i.e. the modulatory effect of Ca2+ on conformational flexibility of RD, is in line with the observed suppression of hydrogen-deuterium exchange rate in the Ca2+-bound form, as well as with results of molecular dynamics calculations. Based on this evidence, we concluded that Ca2+-induced change in structural dynamics of RD is a major factor in Ca2+-mediated regulation of Physarum myosin II activity.
About this Structure
2BL0 is a Protein complex structure of sequences from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
Structural evidence for non-canonical binding of Ca2+ to a canonical EF-hand of a conventional myosin., Debreczeni JE, Farkas L, Harmat V, Hetenyi C, Hajdu I, Zavodszky P, Kohama K, Nyitray L, J Biol Chem. 2005 Dec 16;280(50):41458-64. Epub 2005 Oct 13. PMID:16227209
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