3j4g

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_3j4g| PDB=3j4g | SCENE= }}
+
==Structure of lysozyme solved by MicroED to 2.9 A==
-
===Structure of lysozyme solved by MicroED to 2.9 A===
+
<StructureSection load='3j4g' size='340' side='right' caption='[[3j4g]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_24252878}}
+
== Structural highlights ==
-
 
+
<table><tr><td colspan='2'>[[3j4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J4G FirstGlance]. <br>
-
==Function==
+
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j4g RCSB], [http://www.ebi.ac.uk/pdbsum/3j4g PDBsum]</span></td></tr>
 +
</table>
 +
== Function ==
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We demonstrate that it is feasible to determine high-resolution protein structures by electron crystallography of three-dimensional crystals in an electron cryo-microscope (CryoEM). Lysozyme microcrystals were frozen on an electron microscopy grid, and electron diffraction data collected to 1.7 A resolution. We developed a data collection protocol to collect a full-tilt series in electron diffraction to atomic resolution. A single tilt series contains up to 90 individual diffraction patterns collected from a single crystal with tilt angle increment of 0.1-1 degrees and a total accumulated electron dose less than 10 electrons per angstrom squared. We indexed the data from three crystals and used them for structure determination of lysozyme by molecular replacement followed by crystallographic refinement to 2.9 A resolution. This proof of principle paves the way for the implementation of a new technique, which we name 'MicroED', that may have wide applicability in structural biology. DOI: http://dx.doi.org/10.7554/eLife.01345.001.
 +
 +
Three-dimensional electron crystallography of protein microcrystals.,Shi D, Nannenga BL, Iadanza MG, Gonen T Elife. 2013 Jan 1;2(0). pii: e01345. doi: 10.7554/eLife.01345. PMID:24252878<ref>PMID:24252878</ref>
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[3j4g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4G OCA].
+
</div>
-
==Reference==
+
==See Also==
-
<ref group="xtra">PMID:024252878</ref><references group="xtra"/><references/>
+
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Chick]]
[[Category: Chick]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
-
[[Category: Gonen, T.]]
+
[[Category: Gonen, T]]
-
[[Category: Iadanza, M G.]]
+
[[Category: Iadanza, M G]]
-
[[Category: Nannenga, B L.]]
+
[[Category: Nannenga, B L]]
-
[[Category: Shi, D.]]
+
[[Category: Shi, D]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]

Revision as of 04:57, 25 December 2014

Structure of lysozyme solved by MicroED to 2.9 A

3j4g, resolution 2.90Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3j4g"
Personal tools