2bn6
From Proteopedia
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| - | [[Image:2bn6.gif|left|200px]] | + | [[Image:2bn6.gif|left|200px]] |
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| - | '''P-ELEMENT SOMATIC INHIBITOR PROTEIN''' | + | {{Structure |
| + | |PDB= 2bn6 |SIZE=350|CAPTION= <scene name='initialview01'>2bn6</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''P-ELEMENT SOMATIC INHIBITOR PROTEIN''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BN6 is a [ | + | 2BN6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BN6 OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase., Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K, J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:[http:// | + | Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase., Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K, J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15990112 15990112] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: splicing]] | [[Category: splicing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:04:09 2008'' |
Revision as of 14:04, 20 March 2008
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P-ELEMENT SOMATIC INHIBITOR PROTEIN
Overview
P-element transposition in Drosophila is regulated by tissue-specific alternative splicing of the P-element transposase pre-mRNA. In somatic cells, the P-element somatic inhibitor (PSI) protein binds to exon 3 of the pre-mRNA and recruits U1 small nuclear ribonucleoprotein (snRNP) to the F1 pseudo-splice site. This abrogates binding of U1 snRNP to the genuine 5' splice site, thereby preventing excision of the third intron. Two homologous short sequences, referred to as the A and B boxes, near the C terminus of PSI bind to U1-70k protein within U1 snRNP. We have now mapped the AB box-binding site of U1-70k to a short proline-rich sequence at the C terminus. Our NMR study shows that the B box forms an anti-parallel helical hairpin in which four highly conserved aromatic residues form a cluster on one face of the first helix. This hydrophobic cluster interacts extensively with the proline-rich region of the U1-70k protein.
About this Structure
2BN6 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structural basis of the interaction between P-element somatic inhibitor and U1-70k essential for the alternative splicing of P-element transposase., Ignjatovic T, Yang JC, Butler J, Neuhaus D, Nagai K, J Mol Biol. 2005 Aug 5;351(1):52-65. PMID:15990112
Page seeded by OCA on Thu Mar 20 16:04:09 2008
