2bnx

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[[Image:2bnx.gif|left|200px]]<br /><applet load="2bnx" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2bnx.gif|left|200px]]
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caption="2bnx, resolution 2.40&Aring;" />
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'''CRYSTAL STRUCTURE OF THE DIMERIC REGULATORY DOMAIN OF MOUSE DIAPHANEOUS-RELATED FORMIN (DRF), MDIA1'''<br />
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{{Structure
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|PDB= 2bnx |SIZE=350|CAPTION= <scene name='initialview01'>2bnx</scene>, resolution 2.40&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF THE DIMERIC REGULATORY DOMAIN OF MOUSE DIAPHANEOUS-RELATED FORMIN (DRF), MDIA1'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2BNX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BNX OCA].
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2BNX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BNX OCA].
==Reference==
==Reference==
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Structural basis of Rho GTPase-mediated activation of the formin mDia1., Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK, Mol Cell. 2005 Apr 29;18(3):273-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15866170 15866170]
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Structural basis of Rho GTPase-mediated activation of the formin mDia1., Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK, Mol Cell. 2005 Apr 29;18(3):273-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15866170 15866170]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: structural protein]]
[[Category: structural protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:39:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:04:24 2008''

Revision as of 14:04, 20 March 2008

Image:2bnx.gif


PDB ID 2bnx

Drag the structure with the mouse to rotate
, resolution 2.40Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF THE DIMERIC REGULATORY DOMAIN OF MOUSE DIAPHANEOUS-RELATED FORMIN (DRF), MDIA1


Overview

Diaphanous-related formins (DRFs) regulate dynamics of unbranched actin filaments during cell contraction and cytokinesis. DRFs are autoinhibited through intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a conserved N-terminal regulatory element. Autoinhibition is relieved through binding of the GTPase RhoA to the N-terminal element. We report the crystal structure of the dimeric regulatory domain of the DRF, mDia1. Dimerization is mediated by an intertwined six-helix bundle, from which extend two Diaphanous inhibitory domains (DIDs) composed of five armadillo repeats. NMR and biochemical mapping indicate the RhoA and DAD binding sites on the DID partially overlap, explaining activation of mDia1 by the GTPase. RhoA binding also requires an additional structurally independent segment adjacent to the DID. This regulatory construction, involving a GTPase binding site spanning a flexibly tethered arm and the inhibitory module, is observed in many autoinhibited effectors of Ras superfamily GTPases, suggesting evolutionary pressure for this design.

About this Structure

2BNX is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis of Rho GTPase-mediated activation of the formin mDia1., Otomo T, Otomo C, Tomchick DR, Machius M, Rosen MK, Mol Cell. 2005 Apr 29;18(3):273-81. PMID:15866170

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