1s7z

From Proteopedia

(Difference between revisions)

Revision as of 05:41, 25 December 2014

Structure of Ocr from Bacteriophage T7

Structural highlights

1s7z is a 1 chain structure with sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Gene:	0.3 (Enterobacteria phage T7)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[V03_BPT7] Prevents both degradation and modification of T7 DNA by the host restriction-modification complex. Structural mimic of the phosphate backbone of B-form DNA that binds to and completely occupies the DNA-binding site of all type I restriction/modification enzymes. Thereby, inhibits the restriction endonuclease activity and protects the phage genome as it penetrates into host cytoplasm.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We have solved, by X-ray crystallography to a resolution of 1.8 A, the structure of a protein capable of mimicking approximately 20 base pairs of B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate backbone of B-form DNA. We also demonstrate that ocr is an efficient inhibitor in vivo of all known families of the complex type I DNA restriction enzymes. Using atomic force microscopy, we have also observed that type I enzymes induce a bend in DNA of similar magnitude to the bend in the ocr molecule. This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA.

Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA.,Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT Mol Cell. 2002 Jan;9(1):187-94. PMID:11804597^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Studier FW. Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J Mol Biol. 1975 May 15;94(2):283-95. PMID:1095770
↑ Atanasiu C, Su TJ, Sturrock SS, Dryden DT. Interaction of the ocr gene 0.3 protein of bacteriophage T7 with EcoKI restriction/modification enzyme. Nucleic Acids Res. 2002 Sep 15;30(18):3936-44. PMID:12235377
↑ Walkinshaw MD, Taylor P, Sturrock SS, Atanasiu C, Berge T, Henderson RM, Edwardson JM, Dryden DT. Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA. Mol Cell. 2002 Jan;9(1):187-94. PMID:11804597

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1s7z"

@@ Line 3: / Line 3: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1s7z]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S7Z FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene><br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">0.3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7])</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">0.3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 Enterobacteria phage T7])</td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s7z RCSB], [http://www.ebi.ac.uk/pdbsum/1s7z PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s7z RCSB], [http://www.ebi.ac.uk/pdbsum/1s7z PDBsum]</span></td></tr>
-<table>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/V03_BPT7 V03_BPT7]] Prevents both degradation and modification of T7 DNA by the host restriction-modification complex. Structural mimic of the phosphate backbone of B-form DNA that binds to and completely occupies the DNA-binding site of all type I restriction/modification enzymes. Thereby, inhibits the restriction endonuclease activity and protects the phage genome as it penetrates into host cytoplasm.<ref>PMID:1095770</ref> <ref>PMID:12235377</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 33: @@
 </StructureSection>
 [[Category: Enterobacteria phage t7]]
-[[Category: Atanasiu, C.]]
+[[Category: Atanasiu, C]]
-[[Category: Berg, T.]]
+[[Category: Berg, T]]
-[[Category: Dryden, D T.]]
+[[Category: Dryden, D T]]
-[[Category: Edwardson, J M.]]
+[[Category: Edwardson, J M]]
-[[Category: Henderson, R M.]]
+[[Category: Henderson, R M]]
-[[Category: Sturrock, S S.]]
+[[Category: Sturrock, S S]]
-[[Category: Taylor, P.]]
+[[Category: Taylor, P]]
-[[Category: Walkinshaw, M D.]]
+[[Category: Walkinshaw, M D]]
 [[Category: All helical]]
 [[Category: Gene regulation]]

1s7z

From Proteopedia

Revision as of 05:41, 25 December 2014

Structure of Ocr from Bacteriophage T7

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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