2bt6

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[[Image:2bt6.gif|left|200px]]<br /><applet load="2bt6" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2bt6.gif|left|200px]]
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caption="2bt6, resolution 1.50&Aring;" />
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'''RU(BPY)2(MBPY)-MODIFIED BOVINE ADRENODOXIN'''<br />
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{{Structure
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|PDB= 2bt6 |SIZE=350|CAPTION= <scene name='initialview01'>2bt6</scene>, resolution 1.50&Aring;
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+B'>AC1</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene> and <scene name='pdbligand=RUA:(4'-METHYL-2,2'BIPYRIDINE)BIS(2,2'-BIPYRIDINE)'>RUA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''RU(BPY)2(MBPY)-MODIFIED BOVINE ADRENODOXIN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2BT6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=FES:'>FES</scene> and <scene name='pdbligand=RUA:'>RUA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BT6 OCA].
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2BT6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BT6 OCA].
==Reference==
==Reference==
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Light-induced reduction of bovine adrenodoxin via the covalently bound ruthenium(II) bipyridyl complex: intramolecular electron transfer and crystal structure., Halavaty A, Muller JJ, Contzen J, Jung C, Hannemann F, Bernhardt R, Galander M, Lendzian F, Heinemann U, Biochemistry. 2006 Jan 24;45(3):709-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16411746 16411746]
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Light-induced reduction of bovine adrenodoxin via the covalently bound ruthenium(II) bipyridyl complex: intramolecular electron transfer and crystal structure., Halavaty A, Muller JJ, Contzen J, Jung C, Hannemann F, Bernhardt R, Galander M, Lendzian F, Heinemann U, Biochemistry. 2006 Jan 24;45(3):709-18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16411746 16411746]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: MG]]
[[Category: MG]]
[[Category: RUA]]
[[Category: RUA]]
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[[Category: 2fe-2s]]
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[[Category: 2fe-2]]
[[Category: bovine adrenodoxin]]
[[Category: bovine adrenodoxin]]
[[Category: electron transport]]
[[Category: electron transport]]
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[[Category: transit peptide]]
[[Category: transit peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:41:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:06:24 2008''

Revision as of 14:06, 20 March 2008

Image:2bt6.gif


PDB ID 2bt6

Drag the structure with the mouse to rotate
, resolution 1.50Å
Sites:
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



RU(BPY)2(MBPY)-MODIFIED BOVINE ADRENODOXIN


Overview

Bovine adrenodoxin (Adx) plays an important role in the electron-transfer process in the mitochondrial steroid hydroxylase system of the bovine adrenal cortex. Using electron paramagnetic resonance (EPR) spectroscopy, we showed that photoreduction of the [2Fe-2S] cluster of Adx via (4'-methyl-2,2'-bipyridine)bis(2,2'-bipyridine)ruthenium(II) [Ru(bpy)2(mbpy)] covalently attached to the protein surface can be used as a new approach to probe the "shuttle" hypothesis for the electron transfer by Adx. The 1.5 A resolution crystal structure of a 1:1 Ru(bpy)2(mbpy)-Adx(1-108) complex reveals the site of modification, Cys95, and allows to predict the possible intramolecular electron-transfer pathways within the complex. Photoreduction of uncoupled Adx, mutant Adx(1-108), and Ru(bpy)2(mbpy)-Adx(1-108) using safranin T as the mediating electron donor suggests that two electrons are transferred from the dye to Adx. The intramolecular photoreduction rate constant for the ruthenated Adx has been determined and is discussed according to the predicted pathways.

About this Structure

2BT6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Light-induced reduction of bovine adrenodoxin via the covalently bound ruthenium(II) bipyridyl complex: intramolecular electron transfer and crystal structure., Halavaty A, Muller JJ, Contzen J, Jung C, Hannemann F, Bernhardt R, Galander M, Lendzian F, Heinemann U, Biochemistry. 2006 Jan 24;45(3):709-18. PMID:16411746

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