2bv3
From Proteopedia
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| - | [[Image:2bv3.gif|left|200px]] | + | [[Image:2bv3.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF A MUTANT ELONGATION FACTOR G TRAPPED WITH A GTP ANALOGUE''' | + | {{Structure |
| + | |PDB= 2bv3 |SIZE=350|CAPTION= <scene name='initialview01'>2bv3</scene>, resolution 2.50Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER'>GNP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF A MUTANT ELONGATION FACTOR G TRAPPED WITH A GTP ANALOGUE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BV3 is a [ | + | 2BV3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BV3 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a mutant elongation factor G trapped with a GTP analogue., Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT, FEBS Lett. 2005 Aug 15;579(20):4492-7. PMID:[http:// | + | Crystal structure of a mutant elongation factor G trapped with a GTP analogue., Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT, FEBS Lett. 2005 Aug 15;579(20):4492-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16083884 16083884] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
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[[Category: translation mutation thr84ala]] | [[Category: translation mutation thr84ala]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:07:05 2008'' |
Revision as of 14:07, 20 March 2008
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| , resolution 2.50Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A MUTANT ELONGATION FACTOR G TRAPPED WITH A GTP ANALOGUE
Overview
Elongation factor G (EF-G) is a G protein factor that catalyzes the translocation step in protein synthesis on the ribosome. Its GTP conformation in the absence of the ribosome is currently unknown. We present the structure of a mutant EF-G (T84A) in complex with the non-hydrolysable GTP analogue GDPNP. The crystal structure provides a first insight into conformational changes induced in EF-G by GTP. Comparison of this structure with that of EF-G in complex with GDP suggests that the GTP and GDP conformations in solution are very similar and that the major contribution to the active GTPase conformation, which is quite different, therefore comes from its interaction with the ribosome.
About this Structure
2BV3 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a mutant elongation factor G trapped with a GTP analogue., Hansson S, Singh R, Gudkov AT, Liljas A, Logan DT, FEBS Lett. 2005 Aug 15;579(20):4492-7. PMID:16083884
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