2bvy
From Proteopedia
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| - | [[Image:2bvy.gif|left|200px]] | + | [[Image:2bvy.gif|left|200px]] |
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| - | '''THE STRUCTURE AND CHARACTERIZATION OF A MODULAR ENDO-BETA-1,4-MANNANASE FROM CELLULOMONAS FIMI''' | + | {{Structure |
| + | |PDB= 2bvy |SIZE=350|CAPTION= <scene name='initialview01'>2bvy</scene>, resolution 2.25Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Act+Binding+Site+For+Chain+A'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''THE STRUCTURE AND CHARACTERIZATION OF A MODULAR ENDO-BETA-1,4-MANNANASE FROM CELLULOMONAS FIMI''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BVY is a [ | + | 2BVY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cellulomonas_fimi Cellulomonas fimi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BVY OCA]. |
==Reference== | ==Reference== | ||
| - | The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi., Le Nours J, Anderson L, Stoll D, Stalbrand H, Lo Leggio L, Biochemistry. 2005 Sep 27;44(38):12700-8. PMID:[http:// | + | The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi., Le Nours J, Anderson L, Stoll D, Stalbrand H, Lo Leggio L, Biochemistry. 2005 Sep 27;44(38):12700-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16171384 16171384] |
[[Category: Cellulomonas fimi]] | [[Category: Cellulomonas fimi]] | ||
[[Category: Mannan endo-1,4-beta-mannosidase]] | [[Category: Mannan endo-1,4-beta-mannosidase]] | ||
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[[Category: glycoside hydrolase]] | [[Category: glycoside hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:07:25 2008'' |
Revision as of 14:07, 20 March 2008
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| , resolution 2.25Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Mannan endo-1,4-beta-mannosidase, with EC number 3.2.1.78 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE STRUCTURE AND CHARACTERIZATION OF A MODULAR ENDO-BETA-1,4-MANNANASE FROM CELLULOMONAS FIMI
Overview
The endo-beta-1,4-mannanase from the soil bacterium Cellulomonas fimi is a modular plant cell wall degrading enzyme involved in the hydrolysis of the backbone of mannan, one of the most abundant polysaccharides of the hemicellulosic network in the plant cell wall. The crystal structure of a recombinant truncated endo-beta-1,4-mannanase from C. fimi (CfMan26A-50K) was determined by X-ray crystallography to 2.25 A resolution using the molecular replacement technique. The overall structure of the enzyme consists of a core (beta/alpha)8-barrel catalytic module characteristic of clan GH-A, connected via a linker to an immunoglobulin-like module of unknown function. A complex with the oligosaccharide mannotriose to 2.9 A resolution has also been obtained. Both the native structure and the complex show a cacodylate ion bound at the -1 subsite, while subsites -2, -3, and -4 are occupied by mannotriose in the complex. Enzyme kinetic analysis and the analysis of hydrolysis products from manno-oligosaccharides and mannopentitol suggest five important active-site cleft subsites. CfMan26A-50K has a high affinity -3 subsite with Phe325 as an aromatic platform, which explains the mannose releasing property of the enzyme. Structural differences with the homologous Cellvibrio japonicus beta-1,4-mannanase (CjMan26A) at the -2 and -3 subsites may explain the poor performance of CfMan26A mutants as "glycosynthases".
About this Structure
2BVY is a Single protein structure of sequence from Cellulomonas fimi. Full crystallographic information is available from OCA.
Reference
The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi., Le Nours J, Anderson L, Stoll D, Stalbrand H, Lo Leggio L, Biochemistry. 2005 Sep 27;44(38):12700-8. PMID:16171384
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