4bwa
From Proteopedia
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| - | + | ==PylRS Y306G, Y384F, I405R mutant in complex with adenylated norbornene== | |
| - | + | <StructureSection load='4bwa' size='340' side='right' caption='[[4bwa]], [[Resolution|resolution]] 2.45Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4bwa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2053 Dsm 2053]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BWA FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=N0B:ADENYLATED+NORBORNENE'>N0B</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bw9|4bw9]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyrrolysine--tRNA(Pyl)_ligase Pyrrolysine--tRNA(Pyl) ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.26 6.1.1.26] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4bwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4bwa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4bwa RCSB], [http://www.ebi.ac.uk/pdbsum/4bwa PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA]] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity). | [[http://www.uniprot.org/uniprot/PYLS_METMA PYLS_METMA]] Catalyzes the attachment of pyrrolysine to tRNA(Pyl). Pyrrolysine is a lysine derivative encoded by the termination codon UAG (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Three for two: By using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase. | ||
| - | + | Structural Insights into Incorporation of Norbornene Amino Acids for Click Modification of Proteins.,Schneider S, Gattner MJ, Vrabel M, Flugel V, Lopez-Carrillo V, Prill S, Carell T Chembiochem. 2013 Sep 11. doi: 10.1002/cbic.201300435. PMID:24027216<ref>PMID:24027216</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Pyrrolysyl-tRNA synthetase|Pyrrolysyl-tRNA synthetase]] | *[[Pyrrolysyl-tRNA synthetase|Pyrrolysyl-tRNA synthetase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Dsm 2053]] | [[Category: Dsm 2053]] | ||
| - | [[Category: Carell, T | + | [[Category: Carell, T]] |
| - | [[Category: Fluegel, V | + | [[Category: Fluegel, V]] |
| - | [[Category: Gattner, M J | + | [[Category: Gattner, M J]] |
| - | [[Category: Lopez-Carillo, V | + | [[Category: Lopez-Carillo, V]] |
| - | [[Category: Schneider, S | + | [[Category: Schneider, S]] |
| - | [[Category: Vrabel, M | + | [[Category: Vrabel, M]] |
[[Category: Ligase]] | [[Category: Ligase]] | ||
Revision as of 06:22, 25 December 2014
PylRS Y306G, Y384F, I405R mutant in complex with adenylated norbornene
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Categories: Dsm 2053 | Carell, T | Fluegel, V | Gattner, M J | Lopez-Carillo, V | Schneider, S | Vrabel, M | Ligase
