2bzd
From Proteopedia
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| - | [[Image:2bzd.jpg|left|200px]] | + | [[Image:2bzd.jpg|left|200px]] |
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| - | '''GALACTOSE RECOGNITION BY THE CARBOHYDRATE-BINDING MODULE OF A BACTERIAL SIALIDASE.''' | + | {{Structure |
| + | |PDB= 2bzd |SIZE=350|CAPTION= <scene name='initialview01'>2bzd</scene>, resolution 2.00Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Exo-alpha-sialidase Exo-alpha-sialidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.18 3.2.1.18] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''GALACTOSE RECOGNITION BY THE CARBOHYDRATE-BINDING MODULE OF A BACTERIAL SIALIDASE.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2BZD is a [ | + | 2BZD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Micromonospora_viridifaciens Micromonospora viridifaciens]. This structure supersedes the now removed PDB entry 2BQ9. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BZD OCA]. |
==Reference== | ==Reference== | ||
| - | Galactose recognition by the carbohydrate-binding module of a bacterial sialidase., Newstead SL, Watson JN, Bennet AJ, Taylor G, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1483-91. Epub 2005, Oct 19. PMID:[http:// | + | Galactose recognition by the carbohydrate-binding module of a bacterial sialidase., Newstead SL, Watson JN, Bennet AJ, Taylor G, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1483-91. Epub 2005, Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16239725 16239725] |
[[Category: Exo-alpha-sialidase]] | [[Category: Exo-alpha-sialidase]] | ||
[[Category: Micromonospora viridifaciens]] | [[Category: Micromonospora viridifaciens]] | ||
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[[Category: sialidase]] | [[Category: sialidase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:08:46 2008'' |
Revision as of 14:08, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Exo-alpha-sialidase, with EC number 3.2.1.18 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
GALACTOSE RECOGNITION BY THE CARBOHYDRATE-BINDING MODULE OF A BACTERIAL SIALIDASE.
Overview
Glycoside hydrolases often possess carbohydrate-binding modules (CBMs) in addition to their catalytic domains, which help target the enzymes to appropriate substrates and thereby increase their catalytic efficiency. Sialidases hydrolyse the release of sialic acid from a variety of glycoconjugates and play significant roles in the pathogenesis of a number of important diseases. The sialidase from Micromonospora viridifaciens has a CBM which recognizes galactose. The CBM is linked to the catalytic domain by an immunoglobulin-like domain, resulting in the galactose binding site sitting above the catalytic site, suggesting an interplay between the two sites. By studying nine crystallographically independent structures of the M. viridifaciens sialidase, the relative flexibility of the three domains was analysed. A detailed study is also presented of the recognition of galactose and lactose by the M. viridifaciens CBM. The striking structure of this sialidase suggests a role for the CBM in binding to galactose residues unmasked by the adjacent catalytic site.
About this Structure
2BZD is a Single protein structure of sequence from Micromonospora viridifaciens. This structure supersedes the now removed PDB entry 2BQ9. Full crystallographic information is available from OCA.
Reference
Galactose recognition by the carbohydrate-binding module of a bacterial sialidase., Newstead SL, Watson JN, Bennet AJ, Taylor G, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1483-91. Epub 2005, Oct 19. PMID:16239725
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