1aro

Structural highlights

Function

[RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine. [NAAA_BPT7] Plays an important role in the switch between viral transcription and T7 genome replication. Once produced in sufficient amount, interacts with and inhibits T7 RNA polymerase that becomes unable to produce additional transcripts. This lysozyme-polymerase complex in turn plays an active role in T7 genome replication and packaging.^{[1] [2] [3]} Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.^{[4] [5] [6]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Lysozyme 3D structures
• RNA polymerase

References

1. ↑ Moffatt BA, Studier FW. T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell. 1987 Apr 24;49(2):221-7. PMID:3568126
2. ↑ Zhang X, Studier FW. Mechanism of inhibition of bacteriophage T7 RNA polymerase by T7 lysozyme. J Mol Biol. 1997 May 30;269(1):10-27. PMID:9192997 doi:http://dx.doi.org/10.1006/jmbi.1997.1016
3. ↑ Stano NM, Patel SS. T7 lysozyme represses T7 RNA polymerase transcription by destabilizing the open complex during initiation. J Biol Chem. 2004 Apr 16;279(16):16136-43. Epub 2004 Feb 5. PMID:14764584 doi:http://dx.doi.org/10.1074/jbc.M400139200
4. ↑ Moffatt BA, Studier FW. T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell. 1987 Apr 24;49(2):221-7. PMID:3568126
5. ↑ Zhang X, Studier FW. Mechanism of inhibition of bacteriophage T7 RNA polymerase by T7 lysozyme. J Mol Biol. 1997 May 30;269(1):10-27. PMID:9192997 doi:http://dx.doi.org/10.1006/jmbi.1997.1016
6. ↑ Stano NM, Patel SS. T7 lysozyme represses T7 RNA polymerase transcription by destabilizing the open complex during initiation. J Biol Chem. 2004 Apr 16;279(16):16136-43. Epub 2004 Feb 5. PMID:14764584 doi:http://dx.doi.org/10.1074/jbc.M400139200
7. ↑ Jeruzalmi D, Steitz TA. Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme. EMBO J. 1998 Jul 15;17(14):4101-13. PMID:9670025 doi:10.1093/emboj/17.14.4101