2c1l

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[[Image:2c1l.gif|left|200px]]<br /><applet load="2c1l" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2c1l.gif|left|200px]]
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caption="2c1l, resolution 1.90&Aring;" />
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'''STRUCTURE OF THE BFII RESTRICTION ENDONUCLEASE'''<br />
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{{Structure
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|PDB= 2c1l |SIZE=350|CAPTION= <scene name='initialview01'>2c1l</scene>, resolution 1.90&Aring;
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|SITE= <scene name='pdbsite=AC1:Bct+Binding+Site+For+Chain+A'>AC1</scene>
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|LIGAND= <scene name='pdbligand=TAR:D(-)-TARTARIC+ACID'>TAR</scene>, <scene name='pdbligand=BCT:BICARBONATE+ION'>BCT</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4]
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|GENE=
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}}
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'''STRUCTURE OF THE BFII RESTRICTION ENDONUCLEASE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2C1L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_firmus Bacillus firmus] with <scene name='pdbligand=TAR:'>TAR</scene>, <scene name='pdbligand=BCT:'>BCT</scene>, <scene name='pdbligand=TRS:'>TRS</scene>, <scene name='pdbligand=TLA:'>TLA</scene>, <scene name='pdbligand=SRT:'>SRT</scene>, <scene name='pdbligand=MES:'>MES</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] Known structural/functional Site: <scene name='pdbsite=AC1:Bct+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1L OCA].
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2C1L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_firmus Bacillus firmus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1L OCA].
==Reference==
==Reference==
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Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease., Grazulis S, Manakova E, Roessle M, Bochtler M, Tamulaitiene G, Huber R, Siksnys V, Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15797-802. Epub 2005 Oct 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16247004 16247004]
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Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease., Grazulis S, Manakova E, Roessle M, Bochtler M, Tamulaitiene G, Huber R, Siksnys V, Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15797-802. Epub 2005 Oct 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16247004 16247004]
[[Category: Bacillus firmus]]
[[Category: Bacillus firmus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: restriction endonuclease]]
[[Category: restriction endonuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:43:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:09:33 2008''

Revision as of 14:09, 20 March 2008

Image:2c1l.gif


PDB ID 2c1l

Drag the structure with the mouse to rotate
, resolution 1.90Å
Sites:
Ligands: , , , , , and
Activity: Type II site-specific deoxyribonuclease, with EC number 3.1.21.4
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF THE BFII RESTRICTION ENDONUCLEASE


Overview

Among all restriction endonucleases known to date, BfiI is unique in cleaving DNA in the absence of metal ions. BfiI represents a different evolutionary lineage of restriction enzymes, as shown by its crystal structure at 1.9-A resolution. The protein consists of two structural domains. The N-terminal catalytic domain is similar to Nuc, an EDTA-resistant nuclease from the phospholipase D superfamily. The C-terminal DNA-binding domain of BfiI exhibits a beta-barrel-like structure very similar to the effector DNA-binding domain of the Mg(2+)-dependent restriction enzyme EcoRII and to the B3-like DNA-binding domain of plant transcription factors. BfiI presumably evolved through domain fusion of a DNA-recognition element to a nonspecific nuclease akin to Nuc and elaborated a mechanism to limit DNA cleavage to a single double-strand break near the specific recognition sequence. The crystal structure suggests that the interdomain linker may act as an autoinhibitor controlling BfiI catalytic activity in the absence of a specific DNA sequence. A psi-blast search identified a BfiI homologue in a Mesorhizobium sp. BNC1 bacteria strain, a plant symbiont isolated from an EDTA-rich environment.

About this Structure

2C1L is a Single protein structure of sequence from Bacillus firmus. Full crystallographic information is available from OCA.

Reference

Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease., Grazulis S, Manakova E, Roessle M, Bochtler M, Tamulaitiene G, Huber R, Siksnys V, Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15797-802. Epub 2005 Oct 24. PMID:16247004

Page seeded by OCA on Thu Mar 20 16:09:33 2008

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