2c1x

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[[Image:2c1x.gif|left|200px]]<br /><applet load="2c1x" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:2c1x.gif|left|200px]]
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caption="2c1x, resolution 1.90&Aring;" />
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'''STRUCTURE AND ACTIVITY OF A FLAVONOID 3-O GLUCOSYLTRANSFERASE REVEALS THE BASIS FOR PLANT NATURAL PRODUCT MODIFICATION'''<br />
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{{Structure
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|PDB= 2c1x |SIZE=350|CAPTION= <scene name='initialview01'>2c1x</scene>, resolution 1.90&Aring;
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|SITE= <scene name='pdbsite=AC1:B3p+Binding+Site+For+Chain+A'>AC1</scene>
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|LIGAND= <scene name='pdbligand=UDP:URIDINE-5'-DIPHOSPHATE'>UDP</scene> and <scene name='pdbligand=B3P:2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>B3P</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Flavonol_3-O-glucosyltransferase Flavonol 3-O-glucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.91 2.4.1.91]
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|GENE=
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}}
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'''STRUCTURE AND ACTIVITY OF A FLAVONOID 3-O GLUCOSYLTRANSFERASE REVEALS THE BASIS FOR PLANT NATURAL PRODUCT MODIFICATION'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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2C1X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera] with <scene name='pdbligand=UDP:'>UDP</scene> and <scene name='pdbligand=B3P:'>B3P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Flavonol_3-O-glucosyltransferase Flavonol 3-O-glucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.91 2.4.1.91] Known structural/functional Site: <scene name='pdbsite=AC1:B3p+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1X OCA].
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2C1X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vitis_vinifera Vitis vinifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C1X OCA].
==Reference==
==Reference==
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Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification., Offen W, Martinez-Fleites C, Yang M, Kiat-Lim E, Davis BG, Tarling CA, Ford CM, Bowles DJ, Davies GJ, EMBO J. 2006 Mar 22;25(6):1396-405. Epub 2006 Feb 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16482224 16482224]
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Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification., Offen W, Martinez-Fleites C, Yang M, Kiat-Lim E, Davis BG, Tarling CA, Ford CM, Bowles DJ, Davies GJ, EMBO J. 2006 Mar 22;25(6):1396-405. Epub 2006 Feb 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16482224 16482224]
[[Category: Flavonol 3-O-glucosyltransferase]]
[[Category: Flavonol 3-O-glucosyltransferase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: wine]]
[[Category: wine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:43:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:09:46 2008''

Revision as of 14:09, 20 March 2008

Image:2c1x.gif


PDB ID 2c1x

Drag the structure with the mouse to rotate
, resolution 1.90Å
Sites:
Ligands: and
Activity: Flavonol 3-O-glucosyltransferase, with EC number 2.4.1.91
Coordinates: save as pdb, mmCIF, xml



STRUCTURE AND ACTIVITY OF A FLAVONOID 3-O GLUCOSYLTRANSFERASE REVEALS THE BASIS FOR PLANT NATURAL PRODUCT MODIFICATION


Overview

Glycosylation is a key mechanism for orchestrating the bioactivity, metabolism and location of small molecules in living cells. In plants, a large multigene family of glycosyltransferases is involved in these processes, conjugating hormones, secondary metabolites, biotic and abiotic environmental toxins, to impact directly on cellular homeostasis. The red grape enzyme UDP-glucose:flavonoid 3-O-glycosyltransferase (VvGT1) is responsible for the formation of anthocyanins, the health-promoting compounds which, in planta, function as colourants determining flower and fruit colour and are precursors for the formation of pigmented polymers in red wine. We show that VvGT1 is active, in vitro, on a range of flavonoids. VvGT1 is somewhat promiscuous with respect to donor sugar specificity as dissected through full kinetics on a panel of nine sugar donors. The three-dimensional structure of VvGT1 has also been determined, both in its 'Michaelis' complex with a UDP-glucose-derived donor and the acceptor kaempferol and in complex with UDP and quercetin. These structures, in tandem with kinetic dissection of activity, provide the foundation for understanding the mechanism of these enzymes in small molecule homeostasis.

About this Structure

2C1X is a Single protein structure of sequence from Vitis vinifera. Full crystallographic information is available from OCA.

Reference

Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification., Offen W, Martinez-Fleites C, Yang M, Kiat-Lim E, Davis BG, Tarling CA, Ford CM, Bowles DJ, Davies GJ, EMBO J. 2006 Mar 22;25(6):1396-405. Epub 2006 Feb 16. PMID:16482224

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