This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2c4u
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2c4u.gif|left|200px]] | + | [[Image:2c4u.gif|left|200px]] |
| - | + | ||
| - | '''CRYSTAL STRUCTURE OF THE APO FORM OF THE 5'-FLUORO-5'-DEOXYADENOSINE SYNTHASE ENZYME FROM STREPTOMYCES CATTLEYA''' | + | {{Structure |
| + | |PDB= 2c4u |SIZE=350|CAPTION= <scene name='initialview01'>2c4u</scene>, resolution 2.50Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+C'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Adenosyl-fluoride_synthase Adenosyl-fluoride synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF THE APO FORM OF THE 5'-FLUORO-5'-DEOXYADENOSINE SYNTHASE ENZYME FROM STREPTOMYCES CATTLEYA''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2C4U is a [ | + | 2C4U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4U OCA]. |
==Reference== | ==Reference== | ||
| - | Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:[http:// | + | Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16604208 16604208] |
[[Category: Adenosyl-fluoride synthase]] | [[Category: Adenosyl-fluoride synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 29: | Line 38: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:10:50 2008'' |
Revision as of 14:10, 20 March 2008
| |||||||
| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Adenosyl-fluoride synthase, with EC number 2.5.1.63 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE APO FORM OF THE 5'-FLUORO-5'-DEOXYADENOSINE SYNTHASE ENZYME FROM STREPTOMYCES CATTLEYA
Overview
The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
About this Structure
2C4U is a Single protein structure of sequence from Streptomyces cattleya. Full crystallographic information is available from OCA.
Reference
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208
Page seeded by OCA on Thu Mar 20 16:10:50 2008
