2c5b
From Proteopedia
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| - | [[Image:2c5b.jpg|left|200px]] | + | [[Image:2c5b.jpg|left|200px]] |
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| - | '''X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.''' | + | {{Structure |
| + | |PDB= 2c5b |SIZE=350|CAPTION= <scene name='initialview01'>2c5b</scene>, resolution 2.50Å | ||
| + | |SITE= <scene name='pdbsite=AC1:MET+Binding+Site+For+Chain+C'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MET:METHIONINE'>MET</scene> and <scene name='pdbligand=5F1:5'-FLUORO-2',5'-DIDEOXYADENOSINE'>5F1</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Adenosyl-fluoride_synthase Adenosyl-fluoride synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2C5B is a [ | + | 2C5B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C5B OCA]. |
==Reference== | ==Reference== | ||
| - | Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:[http:// | + | Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16604208 16604208] |
[[Category: Adenosyl-fluoride synthase]] | [[Category: Adenosyl-fluoride synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:11:05 2008'' |
Revision as of 14:11, 20 March 2008
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| , resolution 2.50Å | |||||||
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| Sites: | |||||||
| Ligands: | and | ||||||
| Activity: | Adenosyl-fluoride synthase, with EC number 2.5.1.63 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 2'DEOXY-5'DEOXY-FLUOROADENOSINE.
Overview
The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
About this Structure
2C5B is a Single protein structure of sequence from Streptomyces cattleya. Full crystallographic information is available from OCA.
Reference
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates., Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA, Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208
Page seeded by OCA on Thu Mar 20 16:11:05 2008
