4na1
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | ==Crystal Structure of the second ketosynthase from the bacillaene polyketide synthase== | |
| - | + | <StructureSection load='4na1' size='340' side='right' caption='[[4na1]], [[Resolution|resolution]] 1.95Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4na1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NA1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NA1 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qo3|2qo3]], [[2hg4|2hg4]], [[4na2|4na2]], [[4na3|4na3]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pksJ, pksK, BSU17180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4na1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4na1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4na1 RCSB], [http://www.ebi.ac.uk/pdbsum/4na1 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/PKSJ_BACSU PKSJ_BACSU]] Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.<ref>PMID:16460000</ref> <ref>PMID:17234808</ref> | [[http://www.uniprot.org/uniprot/PKSJ_BACSU PKSJ_BACSU]] Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.<ref>PMID:16460000</ref> <ref>PMID:17234808</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The recently discovered trans-acyltransferase modular polyketide synthases catalyze the biosynthesis of a wide range of bioactive natural products in bacteria. Here we report the structure of the second ketosynthase from the bacillaene trans-acyltransferase polyketide synthase. This 1.95 A resolution structure provides the highest resolution view available of a modular polyketide synthase ketosynthase and reveals a flanking subdomain that is homologous to an ordered linker in cis-acyltransferase modular polyketide synthases. The structure of the cysteine-to-serine mutant of the ketosynthase acylated by its natural substrate provides high-resolution details of how a native polyketide intermediate is bound and helps explain the basis of ketosynthase substrate specificity. The substrate range of the ketosynthase was further investigated by mass spectrometry. | ||
| - | + | A Close Look at a Ketosynthase from a Trans-Acyltransferase Modular Polyketide Synthase.,Gay DC, Gay G, Axelrod AJ, Jenner M, Kohlhaas C, Kampa A, Oldham NJ, Piel J, Keatinge-Clay AT Structure. 2014 Feb 4. pii: S0969-2126(14)00008-2. doi:, 10.1016/j.str.2013.12.016. PMID:24508341<ref>PMID:24508341</ref> | |
| - | + | ||
| - | == | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | + | </div> | |
| - | [[Category: Gay, D C | + | == References == |
| - | [[Category: Gay, G R | + | <references/> |
| - | [[Category: Keatinge-Clay, A T | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Bacsu]] | ||
| + | [[Category: Gay, D C]] | ||
| + | [[Category: Gay, G R]] | ||
| + | [[Category: Keatinge-Clay, A T]] | ||
[[Category: Condensing enzyme fold]] | [[Category: Condensing enzyme fold]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 07:29, 25 December 2014
Crystal Structure of the second ketosynthase from the bacillaene polyketide synthase
| |||||||||||
