2c9k
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:2c9k.gif|left|200px]] | + | [[Image:2c9k.gif|left|200px]] |
| - | + | ||
| - | '''STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION''' | + | {{Structure |
| + | |PDB= 2c9k |SIZE=350|CAPTION= <scene name='initialview01'>2c9k</scene>, resolution 2.8Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Mpd+Binding+Site+For+Chain+A'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2C9K is a [ | + | 2C9K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C9K OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution., Boonserm P, Mo M, Angsuthanasombat C, Lescar J, J Bacteriol. 2006 May;188(9):3391-401. PMID:[http:// | + | Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution., Boonserm P, Mo M, Angsuthanasombat C, Lescar J, J Bacteriol. 2006 May;188(9):3391-401. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16621834 16621834] |
[[Category: Bacillus thuringiensis]] | [[Category: Bacillus thuringiensis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: sporulation]] | [[Category: sporulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:12:39 2008'' |
Revision as of 14:12, 20 March 2008
| |||||||
| , resolution 2.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE FUNCTIONAL FORM OF THE MOSQUITO-LARVICIDAL CRY4AA TOXIN FROM BACILLUS THURINGIENSIS AT 2.8 A RESOLUTION
Overview
The Cry4Aa delta-endotoxin from Bacillus thuringiensis is toxic to larvae of Culex, Anopheles, and Aedes mosquitoes, which are vectors of important human tropical diseases. With the objective of designing modified toxins with improved potency that could be used as biopesticides, we determined the structure of this toxin in its functional form at a resolution of 2.8 angstroms. Like other Cry delta-endotoxins, the activated Cry4Aa toxin consists of three globular domains, a seven-alpha-helix bundle responsible for pore formation (domain I) and the following two other domains having structural similarities with carbohydrate binding proteins: a beta-prism (domain II) and a plant lectin-like beta-sandwich (domain III). We also studied the effect on toxicity of amino acid substitutions and deletions in three loops located at the surface of the putative receptor binding domain II of Cry4Aa. Our results indicate that one loop is an important determinant of toxicity, presumably through attachment of Cry4Aa to the surface of mosquito cells. The availability of the Cry4Aa structure should guide further investigations aimed at the molecular basis of the target specificity and membrane insertion of Cry endotoxins.
About this Structure
2C9K is a Single protein structure of sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA.
Reference
Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution., Boonserm P, Mo M, Angsuthanasombat C, Lescar J, J Bacteriol. 2006 May;188(9):3391-401. PMID:16621834
Page seeded by OCA on Thu Mar 20 16:12:39 2008
