4h63

From Proteopedia

(Difference between revisions)

Revision as of 08:31, 25 December 2014

Structure of the Schizosaccharomyces pombe Mediator head module

Structural highlights

4h63 is a 6 chain structure with sequence from Schizosaccharomyces pombe 972h-. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4h62, 4h61
Gene:	med6, pmc5, SPAC1002.15c (Schizosaccharomyces pombe 972h-), med8, sep15, SPBC21.04 (Schizosaccharomyces pombe 972h-), med11, SPAC644.10 (Schizosaccharomyces pombe 972h-), med17, SPBC31F10.04c, srb4 (Schizosaccharomyces pombe 972h-), med18, pmc6, sep11, SPAC5D6.05 (Schizosaccharomyces pombe 972h-), med22, SPAC29A4.07, srb6 (Schizosaccharomyces pombe 972h-)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[MED11_SCHPO] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex (PIC) with RNA polymerase II and the general transcription factors. The essential med11/22 heterodimer specifically functions in promoting stable PIC formation (By similarity). [MED8_SCHPO] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [MED18_SCHPO] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.^[1]

Publication Abstract from PubMed

Gene transcription by RNA polymerase (Pol) II requires the coactivator complex Mediator. Mediator connects transcriptional regulators and Pol II, and is linked to human disease. Mediator from the yeast Saccharomyces cerevisiae has a molecular mass of 1.4 megadaltons and comprises 25 subunits that form the head, middle, tail and kinase modules. The head module constitutes one-half of the essential Mediator core, and comprises the conserved subunits Med6, Med8, Med11, Med17, Med18, Med20 and Med22. Recent X-ray analysis of the S. cerevisiae head module at 4.3 A resolution led to a partial architectural model with three submodules called neck, fixed jaw and moveable jaw. Here we determine de novo the crystal structure of the head module from the fission yeast Schizosaccharomyces pombe at 3.4 A resolution. Structure solution was enabled by new structures of Med6 and the fixed jaw, and previous structures of the moveable jaw and part of the neck, and required deletion of Med20. The S. pombe head module resembles the head of a crocodile with eight distinct elements, of which at least four are mobile. The fixed jaw comprises tooth and nose domains, whereas the neck submodule contains a helical spine and one limb, with shoulder, arm and finger elements. The arm and the essential shoulder contact other parts of Mediator. The jaws and a central joint are implicated in interactions with Pol II and its carboxy-terminal domain, and the joint is required for transcription in vitro. The S. pombe head module structure leads to a revised model of the S. cerevisiae module, reveals a high conservation and flexibility, explains known mutations, and provides the basis for unravelling a central mechanism of gene regulation.

Structure of the Mediator head module.,Lariviere L, Plaschka C, Seizl M, Wenzeck L, Kurth F, Cramer P Nature. 2012 Oct 31. doi: 10.1038/nature11670. PMID:23123849^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Szilagyi Z, Grallert A, Nemeth N, Sipiczki M. The Schizosaccharomyces pombe genes sep10 and sep11 encode putative general transcriptional regulators involved in multiple cellular processes. Mol Genet Genomics. 2002 Dec;268(4):553-62. Epub 2002 Nov 7. PMID:12471453 doi:http://dx.doi.org/10.1007/s00438-002-0773-3
↑ Lariviere L, Plaschka C, Seizl M, Wenzeck L, Kurth F, Cramer P. Structure of the Mediator head module. Nature. 2012 Oct 31. doi: 10.1038/nature11670. PMID:23123849 doi:http://dx.doi.org/10.1038/nature11670

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4h63"

@@ Line 1: / Line 1: @@
-[[Image:4h63.png|left|200px]]
+==Structure of the Schizosaccharomyces pombe Mediator head module==
+<StructureSection load='4h63' size='340' side='right' caption='[[4h63]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4h63]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H63 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4H63 FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4h62|4h62]], [[4h61|4h61]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">med6, pmc5, SPAC1002.15c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-]), med8, sep15, SPBC21.04 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-]), med11, SPAC644.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-]), med17, SPBC31F10.04c, srb4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-]), med18, pmc6, sep11, SPAC5D6.05 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-]), med22, SPAC29A4.07, srb6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Schizosaccharomyces pombe 972h-])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h63 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h63 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h63 RCSB], [http://www.ebi.ac.uk/pdbsum/4h63 PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/MED11_SCHPO MED11_SCHPO]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex (PIC) with RNA polymerase II and the general transcription factors. The essential med11/22 heterodimer specifically functions in promoting stable PIC formation (By similarity). [[http://www.uniprot.org/uniprot/MED8_SCHPO MED8_SCHPO]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. [[http://www.uniprot.org/uniprot/MED18_SCHPO MED18_SCHPO]] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.<ref>PMID:12471453</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Gene transcription by RNA polymerase (Pol) II requires the coactivator complex Mediator. Mediator connects transcriptional regulators and Pol II, and is linked to human disease. Mediator from the yeast Saccharomyces cerevisiae has a molecular mass of 1.4 megadaltons and comprises 25 subunits that form the head, middle, tail and kinase modules. The head module constitutes one-half of the essential Mediator core, and comprises the conserved subunits Med6, Med8, Med11, Med17, Med18, Med20 and Med22. Recent X-ray analysis of the S. cerevisiae head module at 4.3 A resolution led to a partial architectural model with three submodules called neck, fixed jaw and moveable jaw. Here we determine de novo the crystal structure of the head module from the fission yeast Schizosaccharomyces pombe at 3.4 A resolution. Structure solution was enabled by new structures of Med6 and the fixed jaw, and previous structures of the moveable jaw and part of the neck, and required deletion of Med20. The S. pombe head module resembles the head of a crocodile with eight distinct elements, of which at least four are mobile. The fixed jaw comprises tooth and nose domains, whereas the neck submodule contains a helical spine and one limb, with shoulder, arm and finger elements. The arm and the essential shoulder contact other parts of Mediator. The jaws and a central joint are implicated in interactions with Pol II and its carboxy-terminal domain, and the joint is required for transcription in vitro. The S. pombe head module structure leads to a revised model of the S. cerevisiae module, reveals a high conservation and flexibility, explains known mutations, and provides the basis for unravelling a central mechanism of gene regulation.
-{{STRUCTURE_4h63|  PDB=4h63  |  SCENE=  }}
+Structure of the Mediator head module.,Lariviere L, Plaschka C, Seizl M, Wenzeck L, Kurth F, Cramer P Nature. 2012 Oct 31. doi: 10.1038/nature11670. PMID:23123849<ref>PMID:23123849</ref>
-===Structure of the Schizosaccharomyces pombe Mediator head module===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_23123849}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[4h63]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H63 OCA].
+</StructureSection>
 [[Category: Schizosaccharomyces pombe 972h-]]
-[[Category: Cramer, P.]]
+[[Category: Cramer, P]]
-[[Category: Kurth, F.]]
+[[Category: Kurth, F]]
-[[Category: Lariviere, L.]]
+[[Category: Lariviere, L]]
-[[Category: Plaschka, C.]]
+[[Category: Plaschka, C]]
-[[Category: Seizl, M.]]
+[[Category: Seizl, M]]
-[[Category: Wenzeck, L.]]
+[[Category: Wenzeck, L]]
 [[Category: Mediator complex]]
 [[Category: Nucleus]]
 [[Category: Transcription]]

4h63

From Proteopedia

Revision as of 08:31, 25 December 2014

Structure of the Schizosaccharomyces pombe Mediator head module

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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