3w15
From Proteopedia
(Difference between revisions)
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| - | + | ==Structure of peroxisomal targeting signal 2 (PTS2) of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase in complex with Pex7p and Pex21p== | |
| - | + | <StructureSection load='3w15' size='340' side='right' caption='[[3w15]], [[Resolution|resolution]] 1.80Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[3w15]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bionectria_wenpingii Bionectria wenpingii], [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_s288c Saccharomyces cerevisiae s288c]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3W15 FirstGlance]. <br> | |
| - | ==Function== | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr> |
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PEX7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=466134 Bionectria wenpingii]), PEX21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Saccharomyces cerevisiae S288c]), FOX3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3w15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3w15 RCSB], [http://www.ebi.ac.uk/pdbsum/3w15 PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
[[http://www.uniprot.org/uniprot/PEX7_YEAST PEX7_YEAST]] Binds to the N-terminal PTS2-type peroxisomal targeting signal and plays an essential role in peroxisomal protein import essential for import of 3-oxoacyl-CoA thiolase (a PTS2-containing protein) into peroxisomes. May direct thiolase to peroxisomes by shuttling between the cytosol and peroxisomal membranes. [[http://www.uniprot.org/uniprot/PEX21_YEAST PEX21_YEAST]] Involved in peroxisome biogenesis and the import of peroxisomal matrix proteins that contain the peroxisomal targeting sequence PTS2. Required for peroxisomal targeting of PEX7 and growth on oleate. Acts as an activator of the seryl-tRNA synthetase SES1 by increasing its binding to tRNA.<ref>PMID:9864360</ref> <ref>PMID:12204379</ref> <ref>PMID:12167700</ref> <ref>PMID:17451428</ref> | [[http://www.uniprot.org/uniprot/PEX7_YEAST PEX7_YEAST]] Binds to the N-terminal PTS2-type peroxisomal targeting signal and plays an essential role in peroxisomal protein import essential for import of 3-oxoacyl-CoA thiolase (a PTS2-containing protein) into peroxisomes. May direct thiolase to peroxisomes by shuttling between the cytosol and peroxisomal membranes. [[http://www.uniprot.org/uniprot/PEX21_YEAST PEX21_YEAST]] Involved in peroxisome biogenesis and the import of peroxisomal matrix proteins that contain the peroxisomal targeting sequence PTS2. Required for peroxisomal targeting of PEX7 and growth on oleate. Acts as an activator of the seryl-tRNA synthetase SES1 by increasing its binding to tRNA.<ref>PMID:9864360</ref> <ref>PMID:12204379</ref> <ref>PMID:12167700</ref> <ref>PMID:17451428</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Appropriate targeting of matrix proteins to peroxisomes is mainly directed by two types of peroxisomal targeting signals, PTS1 and PTS2. Although the basis of PTS1 recognition has been revealed by structural studies, that of PTS2 recognition remains elusive. Here we present the crystal structure of a heterotrimeric PTS2-recognition complex from Saccharomyces cerevisiae, containing Pex7p, the C-terminal region of Pex21p and the PTS2 of the peroxisomal 3-ketoacyl-CoA thiolase. Pex7p forms a beta-propeller structure and provides a platform for cooperative interactions with both the amphipathic PTS2 helix and Pex21p. The C-terminal region of Pex21p directly covers the hydrophobic surfaces of both Pex7p and PTS2, and the resulting hydrophobic core is the primary determinant of stable complex formation. Together with in vivo and in vitro functional assays of Pex7p and Pex21p variants, our findings reveal the molecular mechanism of PTS2 recognition. | ||
| - | + | Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p.,Pan D, Nakatsu T, Kato H Nat Struct Mol Biol. 2013 Aug;20(8):987-93. doi: 10.1038/nsmb.2618. Epub 2013 Jun, 30. PMID:23812376<ref>PMID:23812376</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
==See Also== | ==See Also== | ||
*[[Thiolase|Thiolase]] | *[[Thiolase|Thiolase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Bionectria wenpingii]] | [[Category: Bionectria wenpingii]] | ||
| + | [[Category: Ecoli]] | ||
[[Category: Saccharomyces cerevisiae s288c]] | [[Category: Saccharomyces cerevisiae s288c]] | ||
| - | [[Category: Kato, H | + | [[Category: Kato, H]] |
| - | [[Category: Nakatsu, T | + | [[Category: Nakatsu, T]] |
| - | [[Category: Pan, D | + | [[Category: Pan, D]] |
[[Category: Beta-propeller]] | [[Category: Beta-propeller]] | ||
[[Category: Cytosol]] | [[Category: Cytosol]] | ||
Revision as of 09:02, 25 December 2014
Structure of peroxisomal targeting signal 2 (PTS2) of Saccharomyces cerevisiae 3-ketoacyl-CoA thiolase in complex with Pex7p and Pex21p
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